Product Description: high molecular weight rhoptry protein 3
SignalP Peptide: MRSKHLVTLFIITFLSFSTVKVWG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020030 | infected host cell surface knob |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0003723 | RNA binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0044877 | protein-containing complex binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.91 |
| Trophozoite | expressed | 0.54 | 0.74 |
| Schizont | expressed | 3.51 | 0.08 |
| Gametocyte | possibly expressed | 0.16 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0905400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 9 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.173 | MFS: -2.736 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0416000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0416000)
Modification: Tagged | RMgm-4721
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I395
"Best" AlphaFill ligand hit: CTP (cytidine-5'-triphosphate, Local RMSD=5.13) with 7ECD (Global RMSD=22.82)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 27 | 17 | 44 |
| disruptive | 11 | 27 | 22 | 54 |
| missense | 11 | 27 | 21 | 49 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 55 | 42 | 69 |
| disruptive | 18 | 56 | 59 | 124 |
| missense | 15 | 43 | 48 | 100 |
PlasmoDB Total SNPs: 228
Non-coding: 201 | Synonymous: 16 | Nonsynonymous: 10 | Stop Codon: 1
Protein Length: 897 | Molecular Weight (kDa): 104.856
UniProt ID(s): B0M0W0, B0M0W2, B0M159, B0M166, B9VGH3, B9VGH7, B9VGH9, B9VGI1, B9VGI9, B9VGJ0, P11459, Q8I395, Q8WQU1, Q8WQU4
PDB ID(s): None
Isoelectric Point: 6.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 2823136 | A cDNA clone expressing a rhoptry protein of Plasmodium falciparum. | Coppel RL, Bianco AE, ..., Kemp DJ | 10.1016/0166-6851(87)90020-x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21175202 | Proteome analysis reveals a large merozoite surface protein-1 associated complexon the Plasmodium falciparum merozoite surface. | Ranjan R, Chugh M, ..., Malhotra P | 10.1021/pr100875y |
| 25157665 | Human erythrocyte band 3 functions as a receptor for the sialic acid-independentinvasion of Plasmodium falciparum. Role of the RhopH3-MSP1 complex. | Baldwin M, Yamodo I, ..., Chishti AH | 10.1016/j.bbamcr.2014.08.008 |
| 28252383 | Plasmodium falciparum parasites deploy Rhop H2 into the host erythrocyte to obtainnutrients, grow and replicate | Counihan NA, Chisholm SA, ..., de Koning-Ward TF | 10.7554/eLife.23217 |
| 28252384 | The Plasmodium falciparum rhoptry protein Rhop H3 plays essential roles in hostcell invasion and nutrient uptake | Sherling ES, Knuepfer E, ..., van Ooij C | 10.7554/eLife.23239 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 28981210 | Proteome and Structural Organization of the Knob Complex on the Surface of thePlasmodium Infected Red Blood Cell. | Alampalli SV, Grover M, ..., Acharya P | 10.1002/prca.201600177 |
| 29146974 | Human Cyclophilin B forms part of a multi-protein complex during erythrocyteinvasion by Plasmodium falciparum. | Prakash P, Zeeshan M, ..., Malhotra P | 10.1038/s41467-017-01638-6 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33024030 | Phosphorylation of Rhoptry Protein Rhop H3 Is Critical for Host Cell Invasion bythe Malaria Parasite | Ekka R, Gupta A, ..., Sharma P | 10.1128/mBio.00166-20 |
| 33774908 | Characterisation of complexes formed by parasite proteins exported into the hostcell compartment of Plasmodium falciparum infected red blood cells. | Jonsdottir TK, Counihan NA, ..., Gilson PR | 10.1111/cmi.13332 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 35393572 | RhopH2 and RhopH3 export enables assembly of the RhopH complex on P.falciparum-infected erythrocyte membranes. | Pasternak M, Verhoef JMJ, ..., Cowman AF | 10.1038/s42003-022-03290-3 |
| 33393463 | Malaria parasites use a soluble RhopH complex for erythrocyte invasion and anintegral form for nutrient uptake | Schureck MA, Darling JE, ..., Desai SA | 10.7554/eLife.65282 |
| 21320181 | Functional analysis of epigenetic regulation of tandem RhopH1/clag genes revealsa role in Plasmodium falciparum growth | Comeaux CA, Coleman BI, ..., Duraisingh MT | 10.1111/j.1365-2958.2011.07572.x |
| 27299521 | Malaria Parasite CLAG3, a Protein Linked to Nutrient Channels, Participates inHigh Molecular Weight Membrane-Associated Complexes in the Infected Erythrocyte | Zainabadi K | 10.1371/journal.pone.0157390 |
| 34446549 | Native structure of the RhopH complex, a key determinant of malaria parasitenutrient acquisition | Ho CM, Jih J, ..., Zhou ZH | 10.1073/pnas.2100514118 |
| 26415947 | The PfAlba1 RNA-binding protein is an important regulator of translational timingin Plasmodium falciparum blood stages | Vembar SS, Macpherson CR, ..., Scherf A | 10.1186/s13059-015-0771-5 |
| 18508137 | The RhopH complex is transferred to the host cell cytoplasm following red bloodcell invasion by Plasmodium falciparum | Vincensini L, Fall G, ..., Braun Breton C | 10.1016/j.molbiopara.2008.04.002 |
| 25080477 | New antigens for a multicomponent blood-stage malaria vaccine | Osier FH, Mackinnon MJ, ..., Marsh K | 10.1126/scitranslmed.3008705 |
| 32069335 | Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodiumfalciparum genetic cross | Gupta A, Bokhari AAB, ..., Desai SA | 10.1371/journal.ppat.1008363 |
| 28221136 | An essential dual-function complex mediates erythrocyte invasion andchannel-mediated nutrient uptake in malaria parasites | Ito D, Schureck MA, Desai SA | 10.7554/eLife.23485 |
| 32900800 | Live-Cell FRET Reveals that Malaria Nutrient Channel Proteins CLAG3 and RhopH2Remain Associated throughout Their Tortuous Trafficking | Ahmad M, Manzella-Lapeira J, ..., Desai SA | 10.1128/mBio.01354-20 |
| 36753561 | Anti-merozoite antibodies induce natural killer cell effector function and areassociated with immunity against malaria | Odera DO, Tuju J, ..., Williams TN | 10.1126/scitranslmed.abn5993 |
| 18593818 | Identification of Plasmodium falciparum RhopH3 protein peptides that specificallybind to erythrocytes and inhibit merozoite invasion | Pinzon CG, Curtidor H, ..., Patarroyo ME | 10.1110/ps.035923.108 |