PF3D7_0905600 (WDR66)

Genome location: Pf3D7_09_v3:280,207..284,102(+)

Genome classification: Core

Function and Localization

Product Description: WD repeat-containing protein 66, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031514	motile cilium

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.01	1.00
Trophozoite	not expressed	0.02	0.99
Schizont	not expressed	0.11	0.93
Gametocyte	possibly expressed	0.41	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0905600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.771 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I393

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104139

Most Similar Human Ortholog: Q8TBY9

TM-align score: 0.67 | RMSD: 3.80

Seq Identity: 0.18 | Length: 840 / 1220

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000158023	cilia and flagella associated protein 251

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	61	32	71
disruptive	33	163	100	221
missense	28	158	94	207

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	121	47	92
disruptive	63	342	160	306
missense	51	311	140	235

PlasmoDB Total SNPs: 159

Non-coding: 44 | Synonymous: 68 | Nonsynonymous: 46 | Stop Codon: 1

Protein Information

Protein Length: 1220 | Molecular Weight (kDa): 144.252

UniProt ID(s): Q8I393

PDB ID(s): None

Isoelectric Point: 7.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR011047	Quinoprotein alcohol dehydrogenase-like superfamily
PFam	N/A	N/A
Superfamily	SSF50998	Quinoprotein alcohol dehydrogenase-like superfamily