Genome location: Pf3D7_09_v3:298,216..304,767(-)
Genome classification: Core
Product Description: exoribonuclease II
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.27.1 (Transferred entry: 4.6.1.19)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000932 | P-body |
| Component | GO:0000178 | exosome (RNase complex) |
| Component | GO:0034399 | nuclear periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0000175 | 3'-5'-exoribonuclease activity |
| Process | GO:0006402 | mRNA catabolic process |
| Process | GO:0016441 | posttranscriptional gene silencing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.21 | 0.90 |
| Trophozoite | possibly expressed | 0.88 | 0.54 |
| Schizont | expressed | 0.21 | 0.87 |
| Gametocyte | not expressed | 0.51 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0906000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -3.052 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0415300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H520
"Best" AlphaFill ligand hit: U5P (uridine-5'-monophosphate, Local RMSD=0.89) with 7TUV (Global RMSD=4.65)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.27.1 | ribonuclease T2 | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 106 | 56 | 135 |
| disruptive | 67 | 284 | 168 | 361 |
| missense | 44 | 253 | 151 | 305 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 43 | 207 | 74 | 134 |
| disruptive | 124 | 634 | 268 | 480 |
| missense | 84 | 537 | 213 | 360 |
PlasmoDB Total SNPs: 264
Non-coding: 43 | Synonymous: 137 | Nonsynonymous: 84 | Stop Codon: 0
Protein Length: 1797 | Molecular Weight (kDa): 213.841
UniProt ID(s): C0H520
PDB ID(s): None
Isoelectric Point: 9.16
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001900 | Ribonuclease II/R |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR041505 | Dis3-like cold-shock domain 2 |
| PFam | PF00773 | Ribonuclease II/R |
| PFam | PF17849 | Dis3-like cold-shock domain 2 |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25043062 | Exonuclease-mediated degradation of nascent RNA silences genes linked to severemalaria. | Zhang Q, Siegel TN, ..., Scherf A | 10.1038/nature13468 |
| 17380189 | Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function | Murakami H, Goto DB, ..., Yanagida M | 10.1371/journal.pone.0000317 |
| 17242308 | The role of the S1 domain in exoribonucleolytic activity: substrate specificity and multimerization | Amblar M, Barbas A, Gomez-Puertas P, Arraiano CM | 10.1261/rna.220407 |