PF3D7_0906500

Genome location: Pf3D7_09_v3:317,027..319,614(+)

Genome classification: Core

Function and Localization

Product Description: arginase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.5.3.1 (Arginase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005829	cytosol
Function	GO:0004053	arginase activity
Function	GO:0030145	manganese ion binding
Process	GO:0019547	arginine catabolic process to ornithine
Process	GO:0006525	arginine metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.53	0.73
Trophozoite	expressed	0.27	0.84
Schizont	possibly expressed	0.09	0.94
Gametocyte	expressed	0.49	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0906500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.391 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0414800)

Modification: Disrupted | RMgm-4179

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I384

"Best" AlphaFill ligand hit: AG2 ((4-aminobutyl)guanidine, Local RMSD=0.10) with 7LOL (Global RMSD=6.26)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.5.3.1	arginase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P05089	Arginase-1	Homo sapiens	HOGENOM, OMA, OrthoDB	US11389464, Example 1US11389464, Example 3US11389464, Example 4US11389464, Example 5...
Q2KJ64	Arginase-1	Bos taurus	HOGENOM, OMA, OrthoDB	CHEMBL1234777
Q6WVP6	Arginase		HOGENOM, OrthoDB, OrthoMCL	2(S)-amino-6-boronohexanoic acid (ABH)N-hydroxy-L-arginine (NOHA)
P07824	Arginase-1		HOGENOM, OrthoDB	CHEMBL1234777CHEMBL1812661CHEMBL4244287CHEMBL1234777
Q61176	Arginase-1		HOGENOM, OMA, OrthoDB	CHEMBL4639162
P78540	Arginase-2, mitochondrial	Homo sapiens	OrthoDB	US11389464, Example 1US11389464, Example 3US11389464, Example 4US11389464, Example 5...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102008

Most Similar Human Ortholog: P05089

TM-align score: 0.91 | RMSD: 1.69

Seq Identity: 0.32 | Length: 305 / 411

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000081181	arginase 2
ENSG00000118520	arginase 1

Protein Information

Protein Length: 411 | Molecular Weight (kDa): 46.493

UniProt ID(s): Q8I384, Q8WPZ7

PDB ID(s): 3MMR, 3SL0, 3SL1

Isoelectric Point: 5.63

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006035	Ureohydrolase
InterPro	IPR023696	Ureohydrolase domain superfamily
PFam	PF00491	Ureohydrolase
Superfamily	SSF52768	Ureohydrolase domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	22	9	42
disruptive	11	39	24	44
missense	10	35	21	35

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	56	22	27
disruptive	28	84	35	116
missense	22	64	24	69

PlasmoDB Total SNPs: 202

Non-coding: 159 | Synonymous: 33 | Nonsynonymous: 10 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
15843155	Structural metal dependency of the arginase from the human malaria parasitePlasmodium falciparum.	Muller IB, Walter RD, Wrenger C	10.1515/BC.2005.015
20527960	Crystal structure of arginase from Plasmodium falciparum and implications forL-arginine depletion in malarial infection .	Dowling DP, Ilies M, ..., Christianson DW	10.1021/bi100390z