Product Description: ATP-dependent protease ATPase subunit ClpY
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.49 | 0.78 |
| Trophozoite | possibly expressed | 1.35 | 0.35 |
| Schizont | expressed | 1.29 | 0.41 |
| Gametocyte | not expressed | 0.69 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0907400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.208 | MFS: -2.686 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0808900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0808900)
Modification: Disrupted | RMgm-2335
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I377
"Best" AlphaFill ligand hit: TAM (tris(hydroxyethyl)aminomethane, Local RMSD=0.40) with 4L15 (Global RMSD=4.51)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O76031 | ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial | OrthoDB | CHEMBL2047407 |
Protein Length: 922 | Molecular Weight (kDa): 106.462
UniProt ID(s): Q8I377
PDB ID(s): None
Isoelectric Point: 8.47
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR004491 | Heat shock protein HslU |
| InterPro | IPR019489 | Clp ATPase, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF07724 | ATPase, AAA-type, core |
| PFam | PF10431 | Clp ATPase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 47 | 36 | 78 |
| disruptive | 11 | 100 | 73 | 147 |
| missense | 8 | 89 | 70 | 134 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 108 | 58 | 57 |
| disruptive | 32 | 261 | 119 | 213 |
| missense | 21 | 223 | 98 | 149 |
PlasmoDB Total SNPs: 199
Non-coding: 145 | Synonymous: 34 | Nonsynonymous: 20 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22113196 | Disruption of a mitochondrial protease machinery in Plasmodium falciparum is anintrinsic signal for parasite cell death. | Rathore S, Jain S, ..., Mohmmed A | 10.1038/cddis.2011.118 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 30744816 | Specific regions of the SulA protein recognized and degraded by the ATP-dependent ClpYQ (HslUV) protease in Escherichia coli | Chang CY, Weng YT, ..., Wu WF | 10.1016/j.micres.2018.12.003 |
| 29629859 | The ClpY-ClpQ protease regulates multicellular development in Bacillus subtilis | Yu Y, Yan F, ..., Guo JH | 10.1099/mic.0.000658 |
| 27864322 | Escherichia coli Proteome Microarrays Identified the Substrates of ClpYQ Protease | Tsai CH, Ho YH, ..., Chen CS | 10.1074/mcp.M116.065482 |