Product Description: plasma membrane protein 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 3.1.30.1 (Aspergillus nuclease S(1))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005622 | intracellular |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0004519 | endonuclease activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.98 |
| Trophozoite | not expressed | 0.21 | 0.86 |
| Schizont | expressed | 0.47 | 0.70 |
| Gametocyte | not expressed | 0.18 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0908000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.135 | MFS: -2.836 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0809400)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0809400)
Modification: Disrupted | RMgm-4320
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H524
"Best" AlphaFill ligand hit: DCM (2'-deoxycytidine-5'-monophosphate, Local RMSD=0.01) with 5FBF (Global RMSD=4.46)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.30.1 | Aspergillus nuclease S1 | KClSDSEDTAEGTAMgCl25'-AMP5'-GMP5'-ADPKH2PO45'-ATPcitrateheparin... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 35 | 9 | 31 |
| disruptive | 12 | 35 | 21 | 56 |
| missense | 11 | 35 | 19 | 48 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 60 | 18 | 37 |
| disruptive | 23 | 88 | 38 | 120 |
| missense | 18 | 72 | 32 | 85 |
PlasmoDB Total SNPs: 153
Non-coding: 121 | Synonymous: 21 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 416 | Molecular Weight (kDa): 49.356
UniProt ID(s): C0H524
PDB ID(s): None
Isoelectric Point: 9.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003154 | S1/P1 nuclease |
| InterPro | IPR008947 | Phospholipase C/P1 nuclease domain superfamily |
| PFam | PF02265 | S1/P1 nuclease |
| Superfamily | SSF48537 | Phospholipase C/P1 nuclease domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28851956 | A Plasmodium plasma membrane reporter reveals membrane dynamics by live-cellmicroscopy. | Burda PC, Schaffner M, ..., Heussler VT | 10.1038/s41598-017-09569-4 |
| 37700284 | Analysis of acid-tolerance mechanism based on membrane microdomains inSaccharomyces cerevisiae | Lv X, Jin K, ..., Liu L | 10.1186/s12934-023-02195-y |
| 31911490 | RNA-Binding Protein Rnc1 Regulates Cell Length at Division and Acute StressResponse in Fission Yeast through Negative Feedback Modulation of theStress-Activated Mitogen-Activated Protein Kinase Pathway | Prieto-Ruiz F, Vicente-Soler J, ..., Cansado J | 10.1128/mBio.02815-19 |
| 31220665 | Engineering the early secretory pathway for increased protein secretion inSaccharomyces cerevisiae | Besada-Lombana PB, Da Silva NA | 10.1016/j.ymben.2019.06.010 |