PF3D7_0908600

Genome location: Pf3D7_09_v3:398,802..400,941(-)

Genome classification: Core

Function and Localization

Product Description: ribosomal RNA methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.205 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase);2.1.1.56 (mRNA (guanine-N(7)-)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0008173	RNA methyltransferase activity
Function	GO:0008175	tRNA methyltransferase activity
Process	GO:0001510	RNA methylation
Process	GO:0002181	cytoplasmic translation
Process	GO:0030488	tRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.34	0.83
Trophozoite	not expressed	0.34	0.80
Schizont	not expressed	0.05	0.97
Gametocyte	not expressed	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0908600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.164 | MFS: -2.881 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I365

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.71) with 7R7O (Global RMSD=1.99)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.205	tRNA (cytidine32/guanosine34-2'-O)-methyltransferase	No BRENDA inhibitors
2.1.1.56	mRNA (guanine-N7)-methyltransferase	EDTAGpppGThesitribavirinDigitoninsinefunginTriton X-100deoxycholatecycloheximideS-adenosyl-L-cysteineaurintricarboxylic acidS-adenosyl-homocysteine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102125

Most Similar Human Ortholog: Q9UET6

TM-align score: 0.65 | RMSD: 2.38

Seq Identity: 0.44 | Length: 228 / 507

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000068438	FtsJ RNA 2-O-methyltransferase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	32	21	39
disruptive	11	74	37	83
missense	11	74	34	81

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	64	29	43
disruptive	24	157	56	111
missense	22	148	51	91

PlasmoDB Total SNPs: 87

Non-coding: 35 | Synonymous: 28 | Nonsynonymous: 24 | Stop Codon: 0

Protein Information

Protein Length: 507 | Molecular Weight (kDa): 59.696

UniProt ID(s): Q8I365

PDB ID(s): None

Isoelectric Point: 6.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002877	Ribosomal RNA methyltransferase FtsJ domain
InterPro	IPR015507	Ribosomal RNA large subunit methyltransferase E
InterPro	IPR028590	tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF01728	Ribosomal RNA methyltransferase FtsJ domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase