PF3D7_0908700

Genome location: Pf3D7_09_v3:401,013..403,688(-)

Genome classification: Core

Function and Localization

Product Description: tRNA pseudouridine synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0009982	pseudouridine synthase activity
Process	GO:1990481	mRNA pseudouridine synthesis
Process	GO:0031119	tRNA pseudouridine synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.05	0.97
Trophozoite	expressed	0.14	0.91
Schizont	expressed	0.13	0.92
Gametocyte	expressed	0.24	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0908700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.666 | MFS: -1.185 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I0W4

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.4.99.12	tRNA pseudouridine38-40 synthase	tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100424

Most Similar Human Ortholog: Q9BZE2

TM-align score: 0.80 | RMSD: 2.73

Seq Identity: 0.32 | Length: 280 / 312

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000110060	pseudouridine synthase 3
ENSG00000169972	pseudouridine synthase like 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	12	12	21
disruptive	6	41	29	57
missense	6	40	28	54

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	35	21	16
disruptive	10	102	50	77
missense	10	93	43	59

PlasmoDB Total SNPs: 210

Non-coding: 179 | Synonymous: 19 | Nonsynonymous: 10 | Stop Codon: 2

Protein Information

Protein Length: 312 | Molecular Weight (kDa): 37.039

UniProt ID(s): Q8I0W4

PDB ID(s): None

Isoelectric Point: 9.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001406	Pseudouridine synthase I, TruA
InterPro	IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain
InterPro	IPR020103	Pseudouridine synthase, catalytic domain superfamily
PFam	PF01416	Pseudouridine synthase I, TruA, alpha/beta domain
Superfamily	SSF55120	Pseudouridine synthase, catalytic domain superfamily