Product Description: GDP-fucose protein O-fucosyltransferase 2
SignalP Peptide: MKFIIVLLLFFFFKVIDR
# Transmembrane Domains: 0
EC Numbers: 2.4.1.221 (Peptide-O-fucosyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0046922 | peptide-O-fucosyltransferase activity |
| Process | GO:0048870 | cell motility |
| Process | GO:0044409 | entry into host |
| Process | GO:0036066 | protein O-linked fucosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.01 | 0.99 |
| Schizont | not expressed | 0.06 | 0.96 |
| Gametocyte | possibly expressed | 0.17 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0909200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.329 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0810400)
Modification: Disrupted | RMgm-4678
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I360
"Best" AlphaFill ligand hit: GFB (guanosine-5'-diphosphate-beta-l-fucopyranose, Local RMSD=0.22) with 4AP6 (Global RMSD=5.01)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.1.221 | peptide-O-fucosyltransferase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_107032
Most Similar Human Ortholog: Q9Y2G5
TM-align score: 0.76 | RMSD: 2.54
Seq Identity: 0.23 | Length: 354 / 469
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000186866 | protein O-fucosyltransferase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 27 | 21 | 24 |
| disruptive | 8 | 37 | 14 | 58 |
| missense | 8 | 35 | 14 | 55 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 54 | 22 | 32 |
| disruptive | 23 | 73 | 36 | 114 |
| missense | 17 | 61 | 30 | 86 |
PlasmoDB Total SNPs: 92
Non-coding: 77 | Synonymous: 7 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 469 | Molecular Weight (kDa): 57.003
UniProt ID(s): Q8I360
PDB ID(s): None
Isoelectric Point: 9.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR019378 | GDP-fucose protein O-fucosyltransferase |
| PFam | PF10250 | GDP-fucose protein O-fucosyltransferase |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28916755 | Protein O-fucosylation in Plasmodium falciparum ensures efficient infection ofmosquito and vertebrate hosts. | Lopaticki S, Yang ASP, ..., Boddey JA | 10.1038/s41467-017-00571-y |
| 38421807 | Protein O-Fucosyltransferase Is Required for the Efficient Invasion ofHepatocytes by Plasmodium berghei Sporozoites | Srivastava PN, Paul P, Mishra S | 10.1021/acsinfecdis.3c00631 |
| 31334132 | Protein O-Fucosyltransferase 2 Is Not Essential for Plasmodium bergheiDevelopment | Sanz S, Aquilini E, ..., Dinglasan RR | 10.3389/fcimb.2019.00238 |
| 30538131 | O-Fucosylation of thrombospondin-like repeats is required for processing ofmicroneme protein 2 and for efficient host cell invasion by Toxoplasma gondiitachyzoites | Bandini G, Leon DR, ..., Samuelson J | 10.1074/jbc.RA118.005179 |
| 30514763 | Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 isdispensable for Toxoplasma gondii tachyzoite infection | Khurana S, Coffey MJ, ..., Scott NE | 10.1074/jbc.RA118.005357 |