PF3D7_0909900

Genome location: Pf3D7_09_v3:447,302..452,462(+)

Genome classification: Core

Function and Localization

Product Description: helicase SKI2W, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0055087	Ski complex
Function	GO:0003724	RNA helicase activity
Process	GO:0006401	RNA catabolic process
Process	GO:0070478	nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.53	0.75
Trophozoite	not expressed	0.59	0.66
Schizont	not expressed	0.27	0.83
Gametocyte	not expressed	0.51	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0909900

Resistome Missense Mutations: Q777E

Resistome Compounds with Missense Mutations: MMV019017

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.781 | MFS: -3.008 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0811100)

Relative Growth Rate: 0.92 ± 0.13
Confidence: 5.49

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0811100)

Modification: Disrupted | RMgm-2346

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I353

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.17) with 6RO1 (Global RMSD=8.42)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100340

Most Similar Human Ortholog: Q15477

TM-align score: 0.68 | RMSD: 4.76

Seq Identity: 0.33 | Length: 951 / 1373

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000039123	Mtr4 exosome RNA helicase
ENSG00000204351	Ski2 like RNA helicase
ENSG00000206353	Ski2 like RNA helicase
ENSG00000223493	Ski2 like RNA helicase
ENSG00000225737	Ski2 like RNA helicase
ENSG00000228896	Ski2 like RNA helicase
ENSG00000232616	Ski2 like RNA helicase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	84	53	115
disruptive	13	145	92	238
missense	5	126	86	200

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	20	199	77	108
disruptive	48	371	183	428
missense	28	287	145	249

PlasmoDB Total SNPs: 160

Non-coding: 64 | Synonymous: 64 | Nonsynonymous: 32 | Stop Codon: 0

Protein Information

Protein Length: 1373 | Molecular Weight (kDa): 160.373

UniProt ID(s): Q8I353

PDB ID(s): None

Isoelectric Point: 6.97

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR012961	ATP-dependent RNA helicase Ski2, C-terminal
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR016438	ATP-dependent RNA helicase Ski2-like
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF08148	ATP-dependent RNA helicase Ski2, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase