PF3D7_0910500

Genome location: Pf3D7_09_v3:482,386..488,608(-)

Genome classification: Core

Function and Localization

Product Description: DNA repair protein REV1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.5.1.1 (DNA ligase (ATP))

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003887	DNA-directed DNA polymerase activity
Function	GO:0017125	deoxycytidyl transferase activity
Process	GO:0070987	error-free translesion synthesis
Process	GO:0042276	error-prone translesion synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.69	0.67
Trophozoite	not expressed	0.74	0.59
Schizont	possibly expressed	1.22	0.39
Gametocyte	not expressed	0.69	0.62

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0910500

Resistome Missense Mutations: N17D

Resistome Compounds with Missense Mutations: Lopinavir

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.776 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I347

"Best" AlphaFill ligand hit: BAP (1,2,3-trihydroxy-1,2,3,4-tetrahydrobenzo[a]pyrene, Local RMSD=0.03) with 5WM1 (Global RMSD=5.46)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.5.1.1	DNA ligase (ATP)	ATPADPGTPCTPUTPKClNEMEDTANH4+dATPPCNAMgCl2...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_140262

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	85	51	88
disruptive	71	278	163	340
missense	39	236	142	292

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	27	163	62	126
disruptive	151	581	238	433
missense	87	474	198	320

PlasmoDB Total SNPs: 194

Non-coding: 36 | Synonymous: 93 | Nonsynonymous: 65 | Stop Codon: 0

Protein Information

Protein Length: 1697 | Molecular Weight (kDa): 203.097

UniProt ID(s): Q8I347

PDB ID(s): None

Isoelectric Point: 9.3

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001126	UmuC domain
InterPro	IPR001357	BRCT domain
InterPro	IPR036420	BRCT domain superfamily
InterPro	IPR043502	DNA/RNA polymerase superfamily
PFam	PF00817	UmuC domain
PFam	PF16589	BRCT domain
Superfamily	SSF52113	BRCT domain superfamily
Superfamily	SSF56672	DNA/RNA polymerase superfamily