PF3D7_0910700 (ALP5a)

Genome location: Pf3D7_09_v3:491,036..493,173(+)

Genome classification: Core

Function and Localization

Product Description: actin-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005869	dynactin complex

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	not expressed	0.03	0.98
Schizont	possibly expressed	0.07	0.95
Gametocyte	not expressed	0.20	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0910700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.68 | #Insertions: 6

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0811800)

Relative Growth Rate: 1.03 ± 0.07
Confidence: 6.79

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0811800)

Modification: Disrupted | RMgm-2351

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I345

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.46) with 1U2V (Global RMSD=6.64)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532625

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	40	17	42
disruptive	12	60	33	86
missense	9	59	29	84

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	20	72	26	42
disruptive	23	124	65	119
missense	18	110	57	85

PlasmoDB Total SNPs: 86

Non-coding: 42 | Synonymous: 30 | Nonsynonymous: 14 | Stop Codon: 0

Protein Information

Protein Length: 543 | Molecular Weight (kDa): 64.567

UniProt ID(s): Q8I345

PDB ID(s): None

Isoelectric Point: 7.85

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004000	Actin family
InterPro	IPR043129	ATPase, nucleotide binding domain
PFam	PF00022	Actin family
Superfamily	SSF53067	ATPase, nucleotide binding domain