Product Description: cytosolic Fe-S cluster assembly factor NBP35, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0051536 | iron-sulfur cluster binding |
| Process | GO:0016226 | iron-sulfur cluster assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.15 | 0.93 |
| Trophozoite | expressed | 0.53 | 0.69 |
| Schizont | expressed | 0.13 | 0.91 |
| Gametocyte | possibly expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0910800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.422 | MFS: -2.49 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0919700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I344
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.44) with 3KB1 (Global RMSD=2.28)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100169
Most Similar Human Ortholog: Q9Y5Y2
TM-align score: 0.88 | RMSD: 1.94
Seq Identity: 0.41 | Length: 255 / 447
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000095906 | nucleotide binding protein 2 |
| ENSG00000103274 | nucleotide binding protein 1 |
| ENSG00000151413 | nucleotide binding protein like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 30 | 19 | 30 |
| disruptive | 12 | 56 | 38 | 66 |
| missense | 8 | 49 | 38 | 49 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 61 | 22 | 28 |
| disruptive | 26 | 150 | 56 | 135 |
| missense | 17 | 115 | 42 | 61 |
PlasmoDB Total SNPs: 75
Non-coding: 40 | Synonymous: 15 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 447 | Molecular Weight (kDa): 51.002
UniProt ID(s): Q8I344
PDB ID(s): None
Isoelectric Point: 7.5
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000808 | Mrp, conserved site |
| InterPro | IPR019591 | Mrp/NBP35 ATP-binding protein |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR033756 | Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35 |
| PFam | PF10609 | Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35 |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 33222310 | A key cytosolic iron-sulfur cluster synthesis protein localizes to themitochondrion of Toxoplasma gondii. | Aw YTV, Seidi A, ..., van Dooren GG | 10.1111/mmi.14651 |
| 34292549 | Methods to Unravel the Roles of ATPases in Fe-S Cluster Biosynthesis | Mole CN, Dave K, Perlstein DL | 10.1007/978-1-0716-1605-5_9 |
| 37774824 | Interaction between Cfd1 and Nbp35 proteins involved in cytosolic FeS cluster assembly machinery deciphers a stable complexation in Leishmania donovani | Gupta P, Mansuri R, ..., Ali V | 10.1016/j.ijbiomac.2023.127073 |
| 28051091 | Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis | Freibert SA, Goldberg AV, ..., Lill R | 10.1038/ncomms13932 |
| 30679587 | The bacterial Mrp(ORP) is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis | Pardoux R, Fievet A, ..., Aubert C | 10.1038/s41598-018-37021-8 |
| 30483293 | Differential Diel Translation of Transcripts With Roles in the Transfer and Utilization of Iron-Sulfur Clusters in Arabidopsis | Zhang H, Kramer U | 10.3389/fpls.2018.01641 |
| 30201724 | Function and crystal structure of the dimeric P-loop ATPase CFD1 coordinating an exposed [4Fe-4S] cluster for transfer to apoproteins | Stehling O, Jeoung JH, ..., Lill R | 10.1073/pnas.1807762115 |
| 29746244 | Approaches to Interrogate the Role of Nucleotide Hydrolysis by Metal Trafficking NTPases: The Nbp35-Cfd1 Iron-Sulfur Cluster Scaffold as a Case Study | Grossman JD, Camire EJ, Perlstein DL | 10.1016/bs.mie.2017.11.005 |
| 31709520 | The Nbp35/ApbC homolog acts as a nonessential [4Fe-4S] transfer protein inmethanogenic archaea | Zhao C, Lyu Z, ..., Liu Y | 10.1002/1873-3468.13673 |