Product Description: DNA primase large subunit, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005658 | alpha DNA polymerase:primase complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003896 | DNA primase activity |
| Process | GO:0006269 | DNA replication, synthesis of RNA primer |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.29 | 0.81 |
| Schizont | expressed | 0.08 | 0.94 |
| Gametocyte | expressed | 0.19 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0910900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -3.17 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0812000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H531
"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=0.03) with 4UIR (Global RMSD=14.59)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102391
Most Similar Human Ortholog: A0A5F9ZHR0
TM-align score: 0.77 | RMSD: 2.69
Seq Identity: 0.20 | Length: 162 / 539
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000146143 | DNA primase subunit 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 30 | 22 | 33 |
| disruptive | 3 | 23 | 11 | 35 |
| missense | 3 | 23 | 11 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 71 | 20 | 26 |
| disruptive | 7 | 50 | 31 | 130 |
| missense | 5 | 42 | 24 | 81 |
PlasmoDB Total SNPs: 125
Non-coding: 102 | Synonymous: 19 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 539 | Molecular Weight (kDa): 63.945
UniProt ID(s): C0H531
PDB ID(s): None
Isoelectric Point: 9.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR007238 | DNA primase large subunit, eukaryotic/archaeal |
| InterPro | IPR016558 | DNA primase, large subunit, eukaryotic |
| PFam | PF04104 | DNA primase large subunit, eukaryotic/archaeal |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |