PF3D7_0911000

Genome location: Pf3D7_09_v3:499,834..503,245(+)

Genome classification: Core

Function and Localization

Product Description: phosphatidylinositol N-acetylglucosaminyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: 2.4.1.198 (Phosphatidylinositol N-acetylglucosaminyltransferase);3.5.1.89 (N-acetylglucosaminylphosphatidylinositol deacetylase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000506	glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.25	0.84
Schizont	not expressed	0.19	0.87
Gametocyte	not expressed	0.37	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0911000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.534 | MFS: -2.452 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0812100)

Relative Growth Rate: 0.25 ± 0.44
Confidence: 3.03

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H532

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.1.198	phosphatidylinositol N-acetylglucosaminyltransferase	iodoacetic acidN-ethylmaleimideSH-alkylating reagentsp-chloromercuriphenylsulfonic acid
3.5.1.89	N-acetylglucosaminylphosphatidylinositol deacetylase	EDTAethambutolmetaraminolN-hydroxybenzamide2-hydroxybenzoic acidsalicylic hydroxamic acid4-bromo-N-hydroxybenzamide4-bromo-N,2-dihydroxybenzamide5-benzamido-N,2-dihydroxybenzamideN-hydroxy-N'-(4-methoxyphenyl)urea5-amino-N-(benzyloxy)-2-hydroxybenzamide2-(carboxymethyl)-2-deoxy-D-glucopyranose...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103319

Most Similar Human Ortholog: Q92535

TM-align score: 0.84 | RMSD: 2.78

Seq Identity: 0.23 | Length: 278 / 292

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000135845	phosphatidylinositol glycan anchor biosynthesis class C

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	24	7	21
disruptive	1	8	9	27
missense	1	8	9	19

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	36	20	14
disruptive	4	32	27	75
missense	3	20	25	59

PlasmoDB Total SNPs: 217

Non-coding: 200 | Synonymous: 15 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 292 | Molecular Weight (kDa): 33.907

UniProt ID(s): C0H532

PDB ID(s): None

Isoelectric Point: 8.99

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR009450	Phosphatidylinositol N-acetylglucosaminyltransferase subunit C
PFam	PF06432	Phosphatidylinositol N-acetylglucosaminyltransferase subunit C
Superfamily	N/A	N/A