PF3D7_0911700

Genome location: Pf3D7_09_v3:532,079..535,972(+)

Genome classification: Core

Function and Localization

Product Description: GTP-binding protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	expressed	0.64	0.65
Schizont	expressed	0.38	0.78
Gametocyte	not expressed	0.14	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0911700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.845 | MFS: -2.886 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0812800)

Relative Growth Rate: 0.68 ± 0.08
Confidence: 6.34

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0812800)

Modification: Disrupted | RMgm-2357

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I335

"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.02) with 2PMD (Global RMSD=4.01)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100972

Most Similar Human Ortholog: Q8N442

TM-align score: 0.87 | RMSD: 2.05

Seq Identity: 0.38 | Length: 605 / 1085

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000151806	GUF1 homolog, GTPase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	58	25	65
disruptive	21	87	48	125
missense	21	81	45	117

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	100	54	64
disruptive	51	198	72	221
missense	38	170	56	163

PlasmoDB Total SNPs: 173

Non-coding: 30 | Synonymous: 95 | Nonsynonymous: 48 | Stop Codon: 0

Protein Information

Protein Length: 1085 | Molecular Weight (kDa): 126.929

UniProt ID(s): C5J097, C5J099, C5J0A1, C5J0A2, C5J0E3, Q8I335

PDB ID(s): None

Isoelectric Point: 9.06

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000640	Elongation factor EFG, domain V-like
InterPro	IPR000795	Transcription factor, GTP-binding domain
InterPro	IPR005225	Small GTP-binding protein domain
InterPro	IPR009000	Translation protein, beta-barrel domain superfamily
InterPro	IPR013842	GTP-binding protein LepA, C-terminal
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR035647	EF-G domain III/V-like
InterPro	IPR035654	Elongation factor 4, domain IV
PFam	PF00009	Transcription factor, GTP-binding domain
PFam	PF00679	Elongation factor EFG, domain V-like
PFam	PF06421	GTP-binding protein LepA, C-terminal
Superfamily	SSF50447	Translation protein, beta-barrel domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF54980	EF-G domain III/V-like

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895