Product Description: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.220 (tRNA (adenine(58)-N(1))-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0031515 | tRNA (m1A) methyltransferase complex |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.66 | 0.69 |
| Trophozoite | expressed | 0.47 | 0.73 |
| Schizont | possibly expressed | 0.15 | 0.90 |
| Gametocyte | not expressed | 0.27 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0912800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -3.103 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I324
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.220 | tRNA (adenine58-N1)-methyltransferase |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102534
Most Similar Human Ortholog: Q9UJA5
TM-align score: 0.66 | RMSD: 4.42
Seq Identity: 0.21 | Length: 400 / 616
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000089195 | tRNA methyltransferase 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 30 | 18 | 50 |
| disruptive | 50 | 158 | 70 | 141 |
| missense | 21 | 118 | 50 | 106 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 66 | 30 | 57 |
| disruptive | 85 | 281 | 94 | 202 |
| missense | 40 | 195 | 72 | 127 |
PlasmoDB Total SNPs: 161
Non-coding: 70 | Synonymous: 30 | Nonsynonymous: 60 | Stop Codon: 1
Protein Length: 616 | Molecular Weight (kDa): 72.845
UniProt ID(s): Q8I324
PDB ID(s): None
Isoelectric Point: 7.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR017423 | tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 |
| PFam | PF04189 | tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 8628297 | SUI1/p16 is required for the activity of eukaryotic translation initiation factor3 in Saccharomyces cerevisiae | Naranda T, MacMillan SE, Donahue TF, Hershey JW | 10.1128/MCB.16.5.2307 |
| 9660829 | Complex formation by all five homologues of mammalian translation initiationfactor 3 subunits from yeast Saccharomyces cerevisiae | Asano K, Phan L, Anderson J, Hinnebusch AG | 10.1074/jbc.273.29.18573 |
| 10330157 | GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in thematuration of initiator methionyl-tRNA in Saccharomyces cerevisiae | Calvo O, Cuesta R, ..., Tamame M | 10.1128/MCB.19.6.4167 |
| 10811942 | The GCD10 subunit of yeast eIF-3 binds the methyltransferase-like domain of the126 and 183 kDa replicase proteins of tobacco mosaic virus in the yeasttwo-hybrid system | Taylor DN, Carr JP | 10.1099/0022-1317-81-6-1587 |
| 19042972 | Rex1p deficiency leads to accumulation of precursor initiator tRNAMet andpolyadenylation of substrate RNAs in Saccharomyces cerevisiae | Ozanick SG, Wang X, ..., Anderson JT | 10.1093/nar/gkn925 |
| 9223501 | The tobacco mosaic virus RNA polymerase complex contains a plant protein relatedto the RNA-binding subunit of yeast eIF-3 | Osman TA, Buck KW | 10.1128/JVI.71.8.6075-6082.1997 |
| 10779558 | The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine)methyltransferase of Saccharomyces cerevisiae | Anderson J, Phan L, Hinnebusch AG | 10.1073/pnas.090102597 |
| 35901126 | Initiator tRNA lacking 1-methyladenosine is targeted by the rapid tRNA decaypathway in evolutionarily distant yeast species | Tasak M, Phizicky EM | 10.1371/journal.pgen.1010215 |
| 17932071 | Conserved amino acids in each subunit of the heteroligomeric tRNA m1A58 Mtasefrom Saccharomyces cerevisiae contribute to tRNA binding | Ozanick SG, Bujnicki JM, Sem DS, Anderson JT | 10.1093/nar/gkm574 |
| 3540603 | Multiple GCD genes required for repression of GCN4, a transcriptional activatorof amino acid biosynthetic genes in Saccharomyces cerevisiae | Harashima S, Hinnebusch AG | 10.1128/mcb.6.11.3990-3998.1986 |
| 27582183 | Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61from Saccharomyces cerevisiae | Wang M, Zhu Y, ..., Li X | 10.1038/srep32562 |
| 22102254 | Crystallization and preliminary X-ray diffraction crystallographic study of tRNAm(1)A58 methyltransferase from Saccharomyces cerevisiae | Qiu X, Huang K, Ma J, Gao Y | 10.1107/S174430911103733X |