Product Description: 26S proteasome regulatory subunit RPN8, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020020 | food vacuole |
| Component | GO:0000502 | proteasome complex |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.43 | 0.79 |
| Trophozoite | expressed | 0.94 | 0.50 |
| Schizont | expressed | 0.50 | 0.70 |
| Gametocyte | expressed | 0.56 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0912900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.009 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0813900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I323
"Best" AlphaFill ligand hit: ETE (2-{2-[2-2-(methoxy-ethoxy)-ethoxy]-ethoxy}-ethanol, Local RMSD=1.27) with 2O95 (Global RMSD=2.82)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102054
Most Similar Human Ortholog: H3BNT7
TM-align score: 0.93 | RMSD: 1.73
Seq Identity: 0.44 | Length: 175 / 338
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000103035 | proteasome 26S subunit, non-ATPase 7 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 14 | 14 | 27 |
| disruptive | 9 | 15 | 14 | 24 |
| missense | 4 | 9 | 6 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 44 | 22 | 18 |
| disruptive | 16 | 42 | 28 | 65 |
| missense | 5 | 23 | 19 | 37 |
PlasmoDB Total SNPs: 158
Non-coding: 123 | Synonymous: 27 | Nonsynonymous: 7 | Stop Codon: 1
Protein Length: 338 | Molecular Weight (kDa): 39.136
UniProt ID(s): Q8I323
PDB ID(s): None
Isoelectric Point: 8.27
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000555 | JAB1/MPN/MOV34 metalloenzyme domain |
| InterPro | IPR024969 | Rpn11/EIF3F, C-terminal |
| InterPro | IPR033858 | 26S Proteasome non-ATPase regulatory subunit 7/8 |
| PFam | PF01398 | JAB1/MPN/MOV34 metalloenzyme domain |
| PFam | PF13012 | Rpn11/EIF3F, C-terminal |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 22927375 | Near-atomic resolution structural model of the yeast 26S proteasome | Beck F, Unverdorben P, ..., Forster F | 10.1073/pnas.1213333109 |
| 27053109 | The Proteasome Subunit Rpn8 Interacts with the Small Nucleolar RNA Protein(snoRNP) Assembly Protein Pih1 and Mediates Its Ubiquitin-independent Degradationin Saccharomyces cerevisiae | Paci A, Liu PX, Zhang L, Zhao R | 10.1074/jbc.M115.702043 |
| 25631053 | Solution structure of yeast Rpn9: insights into proteasome lid assembly | Hu Y, Wu Y, ..., Jin C | 10.1074/jbc.M114.626762 |