Genome location: Pf3D7_09_v3:574,522..576,690(+)
Genome classification: Core
Product Description: elongation factor 1-beta
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Component | GO:0005853 | eukaryotic translation elongation factor 1 complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005085 | guanyl-nucleotide exchange factor activity |
| Process | GO:0006414 | translational elongation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 1.52 | 0.39 |
| Trophozoite | expressed | 1.92 | 0.22 |
| Schizont | expressed | 0.44 | 0.75 |
| Gametocyte | expressed | 0.88 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0913200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.266 | MFS: -2.711 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0814200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0814200)
Modification: Disrupted | RMgm-2360
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I320
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101695
Most Similar Human Ortholog: F2Z2G2
TM-align score: 0.80 | RMSD: 2.13
Seq Identity: 0.21 | Length: 66 / 276
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000114942 | eukaryotic translation elongation factor 1 beta 2 |
| ENSG00000283391 | eukaryotic translation elongation factor 1 beta 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 9 | 5 | 19 |
| disruptive | 12 | 35 | 25 | 51 |
| missense | 9 | 26 | 19 | 35 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 26 | 16 | 19 |
| disruptive | 19 | 81 | 40 | 76 |
| missense | 13 | 56 | 25 | 48 |
PlasmoDB Total SNPs: 149
Non-coding: 127 | Synonymous: 15 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 276 | Molecular Weight (kDa): 32.027
PDB ID(s): None
Isoelectric Point: 4.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR014038 | Translation elongation factor EF1B, beta/delta subunit, guanine nucleotide exchange domain |
| InterPro | IPR036219 | Translation elongation factor eEF-1beta-like superfamily |
| InterPro | IPR036282 | Glutathione S-transferase, C-terminal domain superfamily |
| PFam | PF00736 | Translation elongation factor EF1B, beta/delta subunit, guanine nucleotide exchange domain |
| Superfamily | SSF47616 | Glutathione S-transferase, C-terminal domain superfamily |
| Superfamily | SSF54984 | Translation elongation factor eEF-1beta-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11251817 | Plasmodium protein phosphatase 2C dephosphorylates translation elongation factor1beta and inhibits its PKC-mediated nucleotide exchange activity in vitro. | Mamoun CB, Goldberg DE | 10.1046/j.1365-2958.2001.02289.x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 17307963 | Purification of components of the translation elongation factor complex ofPlasmodium falciparum by tandem affinity purification. | Takebe S, Witola WH, ..., Ben Mamoun C | 10.1128/EC.00376-06 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 38199565 | Methylation of elongation factor 1A by yeast Efm4 or human eEF1A-KMT2 involves abeta-hairpin recognition motif and crosstalks with phosphorylation | Hamey JJ, Nguyen A, ..., Wilkins MR | 10.1016/j.jbc.2024.105639 |
| 38359077 | Ogataea nonmethanolica f.a, sp. nov., a novel yeast species isolated from rottingwood in Brazil and Colombia | Garcia-Acero AM, Morais CG, ..., Rosa CA | 10.1099/ijsem.0.006273 |