About Gene List

PF3D7_0913300

Genome location: Pf3D7_09_v3:576,759..579,774(-)

Genome classification: Core

Function and Localization

Product Description: conserved protein, unknown function

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.23 (NAD(+) kinase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion
Function GO:0003951 NAD+ kinase activity
Process GO:0019674 NAD metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 0.30 0.86
Trophozoite expressed 0.38 0.77
Schizont expressed 0.08 0.95
Gametocyte possibly expressed 0.14 0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0913300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.192 | MFS: -2.656 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I319

"Best" AlphaFill ligand hit: NAD (nicotinamide-adenine-dinucleotide, Local RMSD=0.35) with 7N29 (Global RMSD=2.91)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.7.1.23 NAD+ kinase ADPAMPKClNEMNAD+NADHPCMBEGTADTNBNADPNADPHNADP+...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105434

Most Similar Human Ortholog: A0A0C4DGV3

TM-align score: 0.72 | RMSD: 3.02

Seq Identity: 0.18 | Length: 209 / 646

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000152620 NAD kinase 2, mitochondrial

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 8 27 19 41
disruptive 26 84 36 90
missense 18 70 28 76

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 13 56 26 48
disruptive 44 154 89 139
missense 31 124 67 89

PlasmoDB Total SNPs: 189

Non-coding: 132 | Synonymous: 32 | Nonsynonymous: 24 | Stop Codon: 1

Protein Information

Protein Length: 646 | Molecular Weight (kDa): 75.847

UniProt ID(s): Q8I319

PDB ID(s): None

Isoelectric Point: 8.49

Protein Domain Annotations:

Source Family ID Description
InterPro IPR016064 NAD kinase/diacylglycerol kinase-like domain superfamily
PFam N/A N/A
Superfamily SSF111331 NAD kinase/diacylglycerol kinase-like domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
34494883 A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. van Esveld SL, Meerstein-Kessel L, ..., Huynen MA 10.1128/mSphere.00614-21