PF3D7_0913500 (AMR4)

Genome location: Pf3D7_09_v3:582,066..584,192(-)

Genome classification: Core

Function and Localization

Product Description: protein AMR4

SignalP Peptide: MIIKFFFFSILFLLYANC

# Transmembrane Domains: 9

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Process	GO:0009657	plastid organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.06	0.97
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.22	0.85
Gametocyte	expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0913500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.381 | MFS: -3.532 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I317

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_112025

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	14	14	22
disruptive	3	22	11	52
missense	3	22	9	48

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	37	16	21
disruptive	17	59	30	74
missense	10	44	27	57

PlasmoDB Total SNPs: 106

Non-coding: 78 | Synonymous: 23 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 432 | Molecular Weight (kDa): 52.13

UniProt ID(s): Q8I317

PDB ID(s): None

Isoelectric Point: 9.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003675	CPBP intramembrane metalloprotease
PFam	PF02517	CPBP intramembrane metalloprotease
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895
30726238	A mutagenesis screen for essential plastid biogenesis genes in human malariaparasites.	Tang Y, Meister TR, ..., Yeh E	10.1371/journal.pbio.3000136
33024034	Caa X-Like Protease of Cyanobacterial Origin Is Required for Complex Plastid Biogenesis in Malaria Parasites	Meister TR, Tang Y, Pulkoski-Gross MJ, Yeh E	10.1128/mBio.01492-20