Product Description: arginine--tRNA ligase, putative
SignalP Peptide: MLNFLILILLFYILFFDECEV
# Transmembrane Domains: 0
EC Numbers: 6.1.1.19 (Arginine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004814 | arginine-tRNA ligase activity |
| Process | GO:0006420 | arginyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.40 | 0.80 |
| Trophozoite | expressed | 0.75 | 0.59 |
| Schizont | possibly expressed | 0.15 | 0.91 |
| Gametocyte | expressed | 0.61 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0913900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.127 | MFS: -2.837 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0814900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I313
"Best" AlphaFill ligand hit: GGB (l-canavanine, Local RMSD=0.38) with 4Q2X (Global RMSD=5.86)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.19 | arginine-tRNA ligase | ADPAMPKClRNAiheminK2SO4ornithinecitrullinediphosphate8-azido-ATPhomoarginineRNA minihelix... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 53 | 28 | 63 |
| disruptive | 32 | 131 | 56 | 150 |
| missense | 29 | 128 | 51 | 144 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 92 | 35 | 73 |
| disruptive | 65 | 241 | 96 | 186 |
| missense | 56 | 215 | 84 | 156 |
PlasmoDB Total SNPs: 159
Non-coding: 73 | Synonymous: 41 | Nonsynonymous: 45 | Stop Codon: 0
Protein Length: 943 | Molecular Weight (kDa): 113.334
UniProt ID(s): Q8I313
PDB ID(s): None
Isoelectric Point: 9.68
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001278 | Arginine-tRNA ligase |
| InterPro | IPR008909 | DALR anticodon binding |
| InterPro | IPR009080 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| InterPro | IPR035684 | Arginyl-tRNA synthetase, catalytic core domain |
| PFam | PF00750 | Arginyl-tRNA synthetase, catalytic core domain |
| PFam | PF05746 | DALR anticodon binding |
| Superfamily | SSF47323 | Aminoacyl-tRNA synthetase, class Ia, anticodon-binding |
| Superfamily | SSF52374 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |