About Gene List

PF3D7_0914000

Genome location: Pf3D7_09_v3:597,575..600,651(+)

Genome classification: Core

Function and Localization

Product Description: pseudouridylate synthase, putative

SignalP Peptide: MFYFLIIFILLTSLLNKC

# Transmembrane Domains: 0

EC Numbers: 4.2.1.70 (Pseudouridylate synthase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0020011 apicoplast
Function GO:0009982 pseudouridine synthase activity
Process GO:0000455 enzyme-directed rRNA pseudouridine synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.21 0.89
Trophozoite expressed 0.37 0.79
Schizont expressed 0.10 0.94
Gametocyte expressed 0.41 0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0914000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.162 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1111000)

  • Relative Growth Rate: 0.91 ± 0.16
  • Confidence: 5.05

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I312

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
4.2.1.70 pseudouridylate synthase tRNAiodoacetamide5-fluorouracilthiol reagents1,10-phenanthrolinep-chloromercuribenzoate1-(Tetrahydro-2-furanyl)-5-fluorouracil

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100211

Most Similar Human Ortholog: H3BRG3

TM-align score: 0.79 | RMSD: 2.84

Seq Identity: 0.17 | Length: 158 / 955

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000007376 RNA pseudouridine synthase domain containing 1
ENSG00000166133 RNA pseudouridine synthase domain containing 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 13 43 29 56
disruptive 106 177 77 191
missense 47 96 51 137

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 21 84 36 63
disruptive 156 292 124 214
missense 70 180 86 140

PlasmoDB Total SNPs: 227

Non-coding: 29 | Synonymous: 85 | Nonsynonymous: 113 | Stop Codon: 0

Protein Information

Protein Length: 955 | Molecular Weight (kDa): 114.23

UniProt ID(s): Q8I312

PDB ID(s): None

Isoelectric Point: 7.31

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006145 Pseudouridine synthase, RsuA/RluA
InterPro IPR006224 Pseudouridine synthase, RluC/RluD, conserved site
InterPro IPR020103 Pseudouridine synthase, catalytic domain superfamily
PFam PF00849 Pseudouridine synthase, RsuA/RluA
Superfamily SSF55120 Pseudouridine synthase, catalytic domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895
31631203 A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. Das D, Krishnan SR, Roy A, Bulusu G 10.1039/c9mo00124g