PF3D7_0914300

Genome location: Pf3D7_09_v3:612,976..615,740(-)

Genome classification: Core

Function and Localization

Product Description: met-10+ like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.228 (tRNA (guanine(37)-N(1))-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005737	cytoplasm
Function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity
Function	GO:0008175	tRNA methyltransferase activity
Process	GO:0002939	tRNA N1-guanine methylation
Process	GO:0030488	tRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.35	0.83
Trophozoite	expressed	0.50	0.71
Schizont	possibly expressed	0.23	0.85
Gametocyte	expressed	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0914300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.616 | #Insertions: 3

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0815300)

Relative Growth Rate: 0.06 ± 0.08
Confidence: 6.54

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H537

"Best" AlphaFill ligand hit: MTA (5'-deoxy-5'-methylthioadenosine, Local RMSD=0.11) with 3A26 (Global RMSD=3.25)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.228	tRNA (guanine37-N1)-methyltransferase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100981

Most Similar Human Ortholog: Q32P41

TM-align score: 0.71 | RMSD: 2.65

Seq Identity: 0.28 | Length: 391 / 781

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000126814	tRNA methyltransferase 5

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	39	21	60
disruptive	36	128	72	172
missense	24	99	60	138

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	81	42	44
disruptive	64	283	143	261
missense	38	225	106	169

PlasmoDB Total SNPs: 123

Non-coding: 37 | Synonymous: 42 | Nonsynonymous: 44 | Stop Codon: 0

Protein Information

Protein Length: 781 | Molecular Weight (kDa): 93.755

UniProt ID(s): C0H537

PDB ID(s): None

Isoelectric Point: 7.94

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type
PFam	PF02475	SAM-dependent methyltransferase TRM5/TYW2-type
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895