About Gene List

PF3D7_0914700 (MFR4)

Genome location: Pf3D7_09_v3:629,371..632,391(+)

Genome classification: Core

Function and Localization

Product Description: major facilitator superfamily-related transporter, putative

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005886 plasma membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.39 0.81
Trophozoite expressed 0.28 0.82
Schizont expressed 0.08 0.95
Gametocyte expressed 0.23 0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0914700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 13 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.707 | #Insertions: 5

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0815700)

Modification: Disrupted | RMgm-1378

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I305

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_124558

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 7 30 27 47
disruptive 15 44 27 63
missense 6 38 25 45

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 16 71 27 49
disruptive 20 114 67 149
missense 8 89 51 87

PlasmoDB Total SNPs: 130

Non-coding: 97 | Synonymous: 16 | Nonsynonymous: 16 | Stop Codon: 1

Protein Information

Protein Length: 516 | Molecular Weight (kDa): 58.421

UniProt ID(s): Q8I305

PDB ID(s): None

Isoelectric Point: 8.33

Protein Domain Annotations:

Source Family ID Description
InterPro IPR011701 Major facilitator superfamily
InterPro IPR036259 MFS transporter superfamily
PFam PF07690 Major facilitator superfamily
Superfamily SSF103473 MFS transporter superfamily

Associated Publications

PMID Title Authors DOI/Link
11152613 Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. Krogh A, Larsson B, von Heijne G, Sonnhammer EL 10.1006/jmbi.2000.4315
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
34756057 Characterization of Apicomplexan Amino Acid Transporters (ApiATs) in the MalariaParasite Plasmodium falciparum. Wichers JS, van Gelder C, ..., Bachmann A 10.1128/mSphere.00743-21