PF3D7_0915200

Genome location: Pf3D7_09_v3:644,974..646,738(+)

Genome classification: Core

Function and Localization

Product Description: ribonuclease H2 subunit C, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.26.4 (Ribonuclease H)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	not expressed	0.23	0.85
Schizont	not expressed	0.05	0.96
Gametocyte	not expressed	0.69	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0915200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -3.048 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0816200)

Relative Growth Rate: 0.41 ± 0.06
Confidence: 6.86

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0816200)

Modification: Disrupted | RMgm-2367

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I300

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.26.4	ribonuclease H	KClNEMSDSDTTNaFDNAEDTAPCMBNH4ClmtSSBDextranmanicol...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533226

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	12	11	30
disruptive	4	49	19	70
missense	4	49	19	66

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	33	17	33
disruptive	17	97	42	100
missense	13	85	39	79

PlasmoDB Total SNPs: 49

Non-coding: 34 | Synonymous: 8 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 322 | Molecular Weight (kDa): 38.672

UniProt ID(s): Q8I300

PDB ID(s): None

Isoelectric Point: 4.91

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013924	Ribonuclease H2, subunit C
PFam	PF08615	Ribonuclease H2, subunit C
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596