Product Description: ATP-dependent 6-phosphofructokinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.11 (6-phosphofructokinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005945 | 6-phosphofructokinase complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0003872 | 6-phosphofructokinase activity |
| Function | GO:0005524 | ATP binding |
| Function | GO:0003723 | RNA binding |
| Function | GO:0008443 | phosphofructokinase activity |
| Process | GO:0009749 | response to glucose |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.24 | 0.49 |
| Trophozoite | expressed | 1.85 | 0.21 |
| Schizont | expressed | 0.54 | 0.68 |
| Gametocyte | expressed | 0.64 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0915400
Resistome Missense Mutations: D106V, L513Q, R860G
Resistome Compounds with Missense Mutations: AN13762, PS-3
Resistome # Samples with Disruptive Mutations: 5 (5 missense, 3 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.165 | MFS: -2.773 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0816400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2Z8
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=1.25) with 1PFK (Global RMSD=2.89)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.11 | 6-phosphofructokinase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O15648 | ATP-dependent 6-phosphofructokinase | Trypanosoma brucei | OrthoMCL BLAST | polysinSuramin hexasodiumCHEMBL3108870CHEMBL3108869... |
Protein Length: 1418 | Molecular Weight (kDa): 159.452
UniProt ID(s): Q8I2Z8
PDB ID(s): None
Isoelectric Point: 6.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000023 | Phosphofructokinase domain |
| InterPro | IPR011183 | Pyrophosphate-dependent phosphofructokinase PfpB |
| InterPro | IPR022953 | ATP-dependent 6-phosphofructokinase |
| InterPro | IPR035966 | Phosphofructokinase superfamily |
| PFam | PF00365 | Phosphofructokinase domain |
| Superfamily | SSF53784 | Phosphofructokinase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 69 | 38 | 131 |
| disruptive | 13 | 60 | 30 | 114 |
| missense | 9 | 60 | 30 | 101 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 192 | 69 | 114 |
| disruptive | 24 | 142 | 62 | 183 |
| missense | 18 | 129 | 53 | 132 |
PlasmoDB Total SNPs: 242
Non-coding: 179 | Synonymous: 41 | Nonsynonymous: 22 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19505469 | Plant-like phosphofructokinase from Plasmodium falciparum belongs to a novelclass of ATP-dependent enzymes. | Mony BM, Mehta M, Jarori GK, Sharma S | 10.1016/j.ijpara.2009.05.011 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33293381 | The Key Glycolytic Enzyme Phosphofructokinase Is Involved in Resistance to Antiplasmodial Glycosides | Fisher GM, Cobbold SA, ..., Andrews KT | 10.1128/mBio.02842-20 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 30425143 | Suppression of Drug Resistance Reveals a Genetic Mechanism of MetabolicPlasticity in Malaria Parasites | Guggisberg AM, Frasse PM, ..., Odom John AR | 10.1128/mBio.01193-18 |