PF3D7_0916600

Genome location: Pf3D7_09_v3:694,527..695,602(-)

Genome classification: Core

Function and Localization

Product Description: methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.64 (3-demethylubiquinol 3-O-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0016279	protein-lysine N-methyltransferase activity
Process	GO:0018027	peptidyl-lysine dimethylation
Process	GO:0018026	peptidyl-lysine monomethylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.11	0.94
Trophozoite	not expressed	0.06	0.96
Schizont	not expressed	0.01	0.99
Gametocyte	not expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0916600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.954 | MFS: -2.435 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0817600)

Relative Growth Rate: 0.98 ± 0.06
Confidence: 6.94

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0817600)

Modification: Disrupted | RMgm-2371

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2Y6

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=1.23) with 6B3A (Global RMSD=5.46)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.-	Ex: homocysteine S-methyltransferase	EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate...
2.1.1.64	3-demethylubiquinol 3-O-methyltransferase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103983

Most Similar Human Ortholog: A0A494BZY7

TM-align score: 0.84 | RMSD: 1.87

Seq Identity: 0.32 | Length: 201 / 219

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000203791	EEF1A lysine methyltransferase 2
ENSG00000258539	novel transcript, METTL10-FAM53B readthrough

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	5	4	20
disruptive	3	20	7	47
missense	3	20	7	42

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	19	10	18
disruptive	5	56	23	92
missense	4	50	20	57

PlasmoDB Total SNPs: 35

Non-coding: 24 | Synonymous: 8 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 219 | Molecular Weight (kDa): 25.927

UniProt ID(s): Q8I2Y6

PDB ID(s): None

Isoelectric Point: 4.37

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR025714	Methyltransferase domain
InterPro	IPR026635	Protein-lysine N-methyltransferase Efm4/METTL10
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF13847	Methyltransferase domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase