About Gene List

PF3D7_0916900 (ZNHIT3)

Genome location: Pf3D7_09_v3:703,682..706,678(+)

Genome classification: Core

Function and Localization

Product Description: Zinc finger HIT domain-containing protein 3

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Component GO:0070761 pre-snoRNP complex
Function GO:0005515 protein binding
Process GO:0000492 box C/D snoRNP assembly
Process GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Process GO:0048254 snoRNA localization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 1.10 0.53
Trophozoite expressed 0.98 0.50
Schizont expressed 1.11 0.45
Gametocyte expressed 0.71 0.62

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0916900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.668 | MFS: -3.103 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2Y4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104054

Most Similar Human Ortholog: A0A087X045

TM-align score: 0.67 | RMSD: 2.07

Seq Identity: 0.38 | Length: 42 / 332

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000273611 zinc finger HIT-type containing 3
ENSG00000278574 zinc finger HIT-type containing 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 2 22 14 16
disruptive 6 76 30 63
missense 6 69 28 58

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 3 34 23 28
disruptive 20 149 39 98
missense 15 128 34 62

PlasmoDB Total SNPs: 202

Non-coding: 183 | Synonymous: 7 | Nonsynonymous: 11 | Stop Codon: 1

Protein Information

Protein Length: 332 | Molecular Weight (kDa): 39.493

UniProt ID(s): Q8I2Y4

PDB ID(s): 7QDW

Isoelectric Point: 4.45

Protein Domain Annotations:

Source Family ID Description
InterPro IPR040197 Zinc finger HIT domain-containing protein 3
PFam N/A N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
35315277 Structural Analysis of the Plasmodial Proteins ZNHIT3 and NUFIP1 ProvidesInsights into the Selectivity of a Conserved Interaction. Chagot ME, Boutilliat A, Kriznik A, Quinternet M 10.1021/acs.biochem.1c00792
35843310 Studies of mutations of assembly factor Hit1 in budding yeast suggest translationdefects as the molecular basis for PEHO syndrome Dreggors-Walker RE, Cohen LN, ..., Ghalei H 10.1016/j.jbc.2022.102261
26210662 Structure/Function Analysis of Protein-Protein Interactions Developed by theYeast Pih1 Platform Protein and Its Partners in Box C/D snoRNP Assembly Quinternet M, Rothe B, ..., Manival X 10.1016/j.jmb.2015.07.012