Product Description: N-glycosylase/DNA lyase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.2.2.- (Hydrolyzing N-glycosyl compounds.);4.2.99.18 (DNA-(apurinic or apyrimidinic site) lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0034039 | 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity |
| Process | GO:0006285 | base-excision repair, AP site formation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.26 | 0.88 |
| Trophozoite | expressed | 0.78 | 0.59 |
| Schizont | expressed | 0.21 | 0.87 |
| Gametocyte | possibly expressed | 0.61 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0917100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.363 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2Y2
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O15527 | N-glycosylase/DNA lyase | OrthoDB | 3,4‐Dichloro‐1‐benzothiophene‐2‐carbohydrazide (08)3‐Chloro‐N'‐[(1Z)‐2‐methylcyclohexylidene]‐1‐benzothiophene‐2‐carbohydrazide (0154)3-Chloro‐N'‐[(1E)‐(4‐fluorophenyl)methylidene]‐1‐... |
Ortholog Group (OrthoMCL): OG6_102483
Most Similar Human Ortholog: O15527
TM-align score: 0.85 | RMSD: 2.76
Seq Identity: 0.29 | Length: 322 / 757
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000114026 | 8-oxoguanine DNA glycosylase |
Protein Length: 757 | Molecular Weight (kDa): 89.361
PDB ID(s): None
Isoelectric Point: 10.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003265 | HhH-GPD domain |
| InterPro | IPR011257 | DNA glycosylase |
| InterPro | IPR012904 | 8-oxoguanine DNA glycosylase, N-terminal |
| PFam | PF00730 | HhH-GPD domain |
| PFam | PF07934 | 8-oxoguanine DNA glycosylase, N-terminal |
| Superfamily | SSF48150 | DNA glycosylase |
| Superfamily | SSF55945 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 37 | 27 | 53 |
| disruptive | 32 | 125 | 88 | 177 |
| missense | 27 | 117 | 84 | 147 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 77 | 44 | 43 |
| disruptive | 63 | 283 | 133 | 214 |
| missense | 46 | 243 | 108 | 140 |
PlasmoDB Total SNPs: 152
Non-coding: 84 | Synonymous: 23 | Nonsynonymous: 45 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 8944766 | Computational method to predict mitochondrially imported proteins and theirtargeting sequences. | Claros MG, Vincens P | 10.1111/j.1432-1033.1996.00779.x |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |