Product Description: pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.51 | 0.76 |
| Trophozoite | expressed | 0.54 | 0.69 |
| Schizont | possibly expressed | 0.08 | 0.95 |
| Gametocyte | not expressed | 0.30 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0917600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.148 | MFS: -2.845 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1446300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2X7
"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.29) with 6ZM2 (Global RMSD=1.98)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100085
Most Similar Human Ortholog: A0A140T8Y5
TM-align score: 0.93 | RMSD: 1.80
Seq Identity: 0.47 | Length: 543 / 820
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000005100 | DEAH-box helicase 33 |
| ENSG00000067596 | DEAH-box helicase 8 |
| ENSG00000101452 | DEAH-box helicase 35 |
| ENSG00000109606 | DEAH-box helicase 15 |
| ENSG00000140829 | DEAH-box helicase 38 |
| ENSG00000204560 | DEAH-box helicase 16 |
| ENSG00000206486 | DEAH-box helicase 16 |
| ENSG00000226171 | DEAH-box helicase 16 |
| ENSG00000227222 | DEAH-box helicase 16 |
| ENSG00000231377 | DEAH-box helicase 16 |
| ENSG00000233049 | DEAH-box helicase 16 |
| ENSG00000233418 | DEAH-box helicase 16 |
| ENSG00000233561 | DEAH-box helicase 16 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 51 | 24 | 65 |
| disruptive | 10 | 48 | 42 | 98 |
| missense | 7 | 48 | 40 | 90 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 107 | 36 | 59 |
| disruptive | 14 | 133 | 61 | 171 |
| missense | 7 | 122 | 58 | 131 |
PlasmoDB Total SNPs: 67
Non-coding: 31 | Synonymous: 19 | Nonsynonymous: 17 | Stop Codon: 0
Protein Length: 820 | Molecular Weight (kDa): 94.145
UniProt ID(s): Q8I2X7
PDB ID(s): None
Isoelectric Point: 7.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR007502 | Helicase-associated domain |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR011709 | Domain of unknown function DUF1605 |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF04408 | Helicase-associated domain |
| PFam | PF07717 | Domain of unknown function DUF1605 |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 31558568 | Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6 | Toroney R, Nielsen KH, Staley JP | 10.1101/gad.328294.119 |
| 37167006 | Conformational dynamics of the RNA binding channel regulates loading and translocation of the DEAH-box helicase Prp43 | Enders M, Ficner R, Adio S | 10.1093/nar/gkad362 |
| 33825710 | The structure of Prp2 bound to RNA and ADP-BeF(3)(-) reveals structural features important for RNA unwinding by DEAH-box ATPases | Hamann F, Zimmerningkat LC, ..., Ficner R | 10.1107/S2059798321001194 |
| 32179686 | Structural basis for DEAH-helicase activation by G-patch proteins | Studer MK, Ivanovic L, ..., Jonas S | 10.1073/pnas.1913880117 |
| 38344068 | Caenorhabditis elegansddx-15 helicase fails to complement loss of Prp43p inSaccharomyces cerevisiae | Karpel JE | 10.17912/micropub.biology.001113 |
| 31092606 | A Non-Dicer RNase III and Four Other Novel Factors Required for RNAi-MediatedTransposon Suppression in the Human Pathogenic Yeast Cryptococcus neoformans | Burke JE, Longhurst AD, ..., Madhani HD | 10.1534/g3.119.400330 |
| 37548918 | Crystal structure of Prp16 in complex with ADP | Garbers TB, Enders M, Neumann P, Ficner R | 10.1107/S2053230X23005721 |