PF3D7_0917700

Genome location: Pf3D7_09_v3:732,608..734,242(+)

Genome classification: Core

Function and Localization

Product Description: GPN-loop GTPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003924	GTPase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.20	0.90
Trophozoite	expressed	0.25	0.84
Schizont	expressed	0.03	0.98
Gametocyte	possibly expressed	0.25	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0917700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.186 | MFS: -3.362 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0818700)

Relative Growth Rate: 0.08 ± 0.11
Confidence: 5.83

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2X6

"Best" AlphaFill ligand hit: GCP (phosphomethylphosphonic acid guanylate ester, Local RMSD=0.30) with 5HCN (Global RMSD=2.95)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102415

Most Similar Human Ortholog: Q9H9Y4

TM-align score: 0.86 | RMSD: 2.21

Seq Identity: 0.41 | Length: 284 / 358

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000142751	GPN-loop GTPase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	16	15	19
disruptive	3	31	25	48
missense	3	31	24	41

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	39	14	27
disruptive	10	86	30	75
missense	8	77	28	50

PlasmoDB Total SNPs: 55

Non-coding: 46 | Synonymous: 4 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 358 | Molecular Weight (kDa): 41.647

UniProt ID(s): Q8I2X6

PDB ID(s): None

Isoelectric Point: 4.54

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004130	GPN-loop GTPase
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR030231	GPN-loop GTPase 2
PFam	PF03029	GPN-loop GTPase
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase