Product Description: glideosome-associated protein 50
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 3.1.3.2 (Acid phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0070258 | inner membrane pellicle complex |
| Component | GO:0005634 | nucleus |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003993 | acid phosphatase activity |
| Function | GO:0008199 | ferric iron binding |
| Function | GO:0008198 | ferrous iron binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0043495 | protein-membrane adaptor activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.70 | 0.63 |
| Schizont | expressed | 1.77 | 0.28 |
| Gametocyte | expressed | 0.61 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0918000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.864 | MFS: -2.249 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0819000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2X3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.2 | acid phosphatase | ATPKClNEMSDSGMPNaFCysIClEDTANH4+PCMBEGTA... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101856
Most Similar Human Ortholog: P13686
TM-align score: 0.82 | RMSD: 1.81
Seq Identity: 0.26 | Length: 281 / 396
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102575 | acid phosphatase 5, tartrate resistant |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 1 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 191
Non-coding: 180 | Synonymous: 7 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 396 | Molecular Weight (kDa): 44.604
PDB ID(s): 3TGH
Isoelectric Point: 8.82
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR024927 | Purple acid phosphatase |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| Superfamily | SSF56300 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16321976 | A conserved molecular motor drives cell invasion and gliding motility acrossmalaria life cycle stages and other apicomplexan parasites. | Baum J, Richard D, ..., Cowman AF | 10.1074/jbc.M509807200 |
| 19561073 | A novel family of Apicomplexan glideosome-associated proteins with an innermembrane-anchoring role. | Bullen HE, Tonkin CJ, ..., Gilson PR | 10.1074/jbc.M109.036772 |
| 19576251 | Effects of calcium signaling on Plasmodium falciparum erythrocyte invasion andpost-translational modification of gliding-associated protein 45 (PfGAP45). | Jones ML, Cottingham C, Rayner JC | 10.1016/j.molbiopara.2009.06.007 |
| 20070315 | Secretion of an acid phosphatase provides a possible mechanism to acquire hostnutrients by Plasmodium falciparum. | Muller IB, Knockel J, ..., Wrenger C | 10.1111/j.1462-5822.2010.01426.x |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 21239623 | Tracking Glideosome-associated protein 50 reveals the development andorganization of the inner membrane complex of Plasmodium falciparum. | Yeoman JA, Hanssen E, ..., Tilley L | 10.1128/EC.00244-10 |
| 22328505 | Origin, composition, organization and function of the inner membrane complex ofPlasmodium falciparum gametocytes. | Dearnley MK, Yeoman JA, ..., Dixon MW | 10.1242/jcs.099002 |
| 22387043 | Crystal structure of GAP50, the anchor of the invasion machinery in the innermembrane complex of Plasmodium falciparum. | Bosch J, Paige MH, ..., Hol WG | 10.1016/j.jsb.2012.02.009 |
| 23332154 | Malaria parasites co-opt human factor H to prevent complement-mediated lysis inthe mosquito midgut. | Simon N, Lasonder E, ..., Pradel G | 10.1016/j.chom.2012.11.013 |
| 25802338 | The Plasmodium Class XIV Myosin, MyoB, Has a Distinct Subcellular Location inInvasive and Motile Stages of the Malaria Parasite and an Unusual Light Chain. | Yusuf NA, Green JL, ..., Holder AA | 10.1074/jbc.M115.637694 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 28985225 | Disrupting assembly of the inner membrane complex blocks Plasmodium falciparumsexual stage development. | Parkyn Schneider M, Liu B, ..., Dixon MWA | 10.1371/journal.ppat.1006659 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 29138437 | Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the innermembrane complex and is required for Plasmodium parasite development | Saini E, Zeeshan M, ..., Tewari R | 10.1038/s41598-017-15781-z |
| 25393004 | Plasticity between MyoC- and MyoA-glideosomes: an example of functionalcompensation in Toxoplasma gondii invasion | Frenal K, Marq JB, ..., Soldati-Favre D | 10.1371/journal.ppat.1004504 |
| 37867646 | Identification of Novel, Potent, and Selective Compounds against Malaria UsingGlideosomal-Associated Protein 50 as a Drug Target | Agrawal P, Kumari S, ..., Sahal D | 10.1021/acsomega.3c05323 |
| 22479457 | Subcellular location, phosphorylation and assembly into the motor complex ofGAP45 during Plasmodium falciparum schizont development | Ridzuan MA, Moon RW, ..., Green JL | 10.1371/journal.pone.0033845 |
| 29150238 | A T Cell Receptor Locus Harbors a Malaria-Specific Immune Response Gene | Van Braeckel-Budimir N, Gras S, ..., Harty JT | 10.1016/j.immuni.2017.10.013 |
| 27113469 | Defining rules of CD8(+) T cell expansion against pre-erythrocytic Plasmodiumantigens in sporozoite-immunized mice | Billman ZP, Kas A, Stone BC, Murphy SC | 10.1186/s12936-016-1295-5 |
| 26845335 | Gliding Associated Proteins Play Essential Roles during the Formation of theInner Membrane Complex of Toxoplasma gondii | Harding CR, Egarter S, ..., Meissner M | 10.1371/journal.ppat.1005403 |
| 20951968 | Functional dissection of the apicomplexan glideosome molecular architecture | Frenal K, Polonais V, ..., Soldati-Favre D | 10.1016/j.chom.2010.09.002 |
| 19047362 | GAP45 phosphorylation controls assembly of the Toxoplasma myosin XIV complex | Gilk SD, Gaskins E, Ward GE, Beckers CJ | 10.1128/EC.00201-08 |
| 17553576 | Identification of protein complexes in detergent-resistant membranes ofPlasmodium falciparum schizonts | Sanders PR, Cantin GT, ..., Crabb BS | 10.1016/j.molbiopara.2007.04.013 |
| 28428983 | Rapid inducible protein displacement in Plasmodiumin vivo and in vitro usingknocksideways technology | Hughes KR, Waters AP | 10.12688/wellcomeopenres.11005.1 |
| 30130043 | [Research Advances on Gliding-associated Proteins of Toxoplasma gondii] | Li RH, Yin GR | https://pubmed.ncbi.nlm.nih.gov/30130043/ |
| 37249423 | Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion andGametocytogenesis | He L, Qiu Y, ..., Zhu X | 10.1128/spectrum.01434-23 |
| 21610105 | Unusual N-glycan structures required for trafficking Toxoplasma gondii GAP50 tothe inner membrane complex regulate host cell entry through parasite motility | Fauquenoy S, Hovasse A, ..., Tomavo S | 10.1074/mcp.M111.008953 |
| 24779470 | Assessment of phosphorylation in Toxoplasma glideosome assembly and function | Jacot D, Frenal K, ..., Soldati-Favre D | 10.1111/cmi.12307 |