Product Description: ATP-dependent DNA helicase Q1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005694 | chromosome |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0043138 | 3'-5' DNA helicase activity |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003677 | DNA binding |
| Function | GO:0009378 | four-way junction helicase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0006310 | DNA recombination |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0000724 | double-strand break repair via homologous recombination |
| Process | GO:0097551 | mitochondrial double-strand break repair |
| Process | GO:0040029 | regulation of gene expression, epigenetic |
| Process | GO:0000723 | telomere maintenance |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.47 | 0.71 |
| Schizont | expressed | 0.17 | 0.89 |
| Gametocyte | expressed | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0918600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.756 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0819500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0819500)
Modification: Disrupted | RMgm-2382
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2W7
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.03) with 6L5M (Global RMSD=2.98)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q14191 | Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN | Homo sapiens | OrthoDB | CHEMBL2426168CHEMBL1374426MLS000559245CHEMBL2426190... |
| P54132 | RecQ-like DNA helicase BLM | Homo sapiens | OrthoDB | CHEMBL2426168MLS000559245CHEMBL2426200CHEMBL2426179... |
Ortholog Group (OrthoMCL): OG6_100352
Most Similar Human Ortholog: P46063
TM-align score: 0.64 | RMSD: 3.55
Seq Identity: 0.33 | Length: 452 / 728
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000004700 | RecQ like helicase |
| ENSG00000197299 | BLM RecQ like helicase |
Protein Length: 728 | Molecular Weight (kDa): 85.509
UniProt ID(s): A0A088SII8, Q8I2W7
PDB ID(s): None
Isoelectric Point: 9.04
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032284 | ATP-dependent DNA helicase RecQ, zinc-binding domain |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| PFam | PF16124 | ATP-dependent DNA helicase RecQ, zinc-binding domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 41 | 24 | 54 |
| disruptive | 24 | 95 | 68 | 123 |
| missense | 12 | 78 | 57 | 108 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 81 | 39 | 51 |
| disruptive | 47 | 222 | 109 | 187 |
| missense | 26 | 168 | 91 | 113 |
PlasmoDB Total SNPs: 108
Non-coding: 50 | Synonymous: 35 | Nonsynonymous: 22 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25111966 | Characterization of recombinant malarial RecQ DNA helicase. | Suntornthiticharoen P, Srila W, ..., Yamabhai M | https://pubmed.ncbi.nlm.nih.gov/25111966/ |
| 26917473 | Plasmodium falciparum Bloom homologue, a nucleocytoplasmic protein, translocatesin 3' to 5' direction and is essential for parasite growth. | Rahman F, Tarique M, Tuteja R | 10.1016/j.bbapap.2016.02.016 |
| 29965959 | RecQ helicases in the malaria parasite Plasmodium falciparum affect genomestability, gene expression patterns and DNA replication dynamics. | Claessens A, Harris LM, ..., Merrick CJ | 10.1371/journal.pgen.1007490 |
| 30728298 | DNA helicase RecQ1 regulates mutually exclusive expression of virulence genes inPlasmodium falciparum via heterochromatin alteration. | Li Z, Yin S, ..., Jiang L | 10.1073/pnas.1811766116 |
| 33239368 | Elucidation of D NA Repair Function of Pf Blm and Potentiation of Artemisinin Action by a Small-Molecule Inhibitor of Rec Q Helicase | Suthram N, Padhi S, ..., Bhattacharyya MK | 10.1128/mSphere.00956-20 |
| 33241842 | Elucidation of an essential function of the unique charged domain of Plasmodiumtopoisomerase I II | Bansod S, Bung N, ..., Bhattacharyya S | 10.1042/BCJ20200318 |
| 34730376 | Bloom Helicase Along with Recombinase Rad51 Repairs the Mitochondrial Genome ofthe Malaria Parasite. | Jha P, Gahlawat A, ..., Bhattacharyya MK | 10.1128/mSphere.00718-21 |