PF3D7_0918800

Genome location: Pf3D7_09_v3:772,161..774,937(-)

Genome classification: Core

Function and Localization

Product Description: dihydrouridine synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 1.3.1.42 (12-oxophytodienoate reductase);1.3.1.89 (tRNA-dihydrouridine(47) synthase (NAD(P)(+)))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.57	0.73
Trophozoite	expressed	0.53	0.71
Schizont	possibly expressed	0.08	0.95
Gametocyte	not expressed	0.23	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0918800

Resistome Missense Mutations: N526D

Resistome Compounds with Missense Mutations: MMV085203

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.719 | #Insertions: 3

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0819700)

Relative Growth Rate: 0.75 ± 0.14
Confidence: 5.26

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0819700)

Modification: Disrupted | RMgm-2383

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2W5

"Best" AlphaFill ligand hit: FNR (two electron reduced flavin mononucleotide, Local RMSD=0.13) with 4XP7 (Global RMSD=2.47)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.3.1.42	12-oxophytodienoate reductase	cortisoneprogesteronep-Hydroxybenzaldehyde
1.3.1.89	tRNA-dihydrouridine47 synthase [NAD(P)+]	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106301

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	33	21	43
disruptive	9	42	34	82
missense	5	33	27	67

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	68	31	34
disruptive	23	112	93	150
missense	11	78	81	113

PlasmoDB Total SNPs: 93

Non-coding: 71 | Synonymous: 9 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 577 | Molecular Weight (kDa): 67.204

UniProt ID(s): Q8I2W5

PDB ID(s): None

Isoelectric Point: 9.64

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001269	tRNA-dihydrouridine synthase
InterPro	IPR004653	tRNA-dihydrouridine(20/20a) synthase
InterPro	IPR018517	tRNA-dihydrouridine synthase, conserved site
InterPro	IPR035587	DUS-like, FMN-binding domain
PFam	PF01207	tRNA-dihydrouridine synthase
Superfamily	SSF51395	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895