PF3D7_0919200

Genome location: Pf3D7_09_v3:788,717..791,151(-)

Genome classification: Core

Function and Localization

Product Description: PPPDE peptidase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0101005	deubiquitinase activity
Process	GO:0016579	protein deubiquitination
Process	GO:0070646	protein modification by small protein removal

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.27	0.90
Trophozoite	expressed	0.06	0.97
Schizont	expressed	0.65	0.75
Gametocyte	possibly expressed	0.29	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0919200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -2.892 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0820100)

Relative Growth Rate: 1.02 ± 0.07
Confidence: 6.63

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0820100)

Modification: Disrupted | RMgm-2385

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2W1

"Best" AlphaFill ligand hit: PH2 (2-amino-6-hydroxymethyl-7,8-dihydro-3h-pteridin-4-one, Local RMSD=0.73) with 6JWU (Global RMSD=19.13)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101256

Most Similar Human Ortholog: B1APK7

TM-align score: 0.59 | RMSD: 1.33

Seq Identity: 0.29 | Length: 65 / 250

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000121644	desumoylating isopeptidase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	6	7	20
disruptive	1	6	8	21
missense	1	6	7	18

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	20	5	23
disruptive	4	27	22	42
missense	2	19	16	36

PlasmoDB Total SNPs: 161

Non-coding: 145 | Synonymous: 16 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 250 | Molecular Weight (kDa): 29.331

UniProt ID(s): Q8I2W1

PDB ID(s): None

Isoelectric Point: 8.57

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008580	PPPDE peptidase domain
PFam	PF05903	PPPDE peptidase domain
Superfamily	N/A	N/A