Genome location: Pf3D7_09_v3:795,265..798,335(+)
Genome classification: Core
Product Description: protein disulfide-isomerase PDI-Trans
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 5.3.4.1 (Protein disulfide-isomerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0003756 | protein disulfide isomerase activity |
| Process | GO:0006457 | protein folding |
| Process | GO:0034976 | response to endoplasmic reticulum stress |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.37 | 0.78 |
| Schizont | expressed | 0.29 | 0.81 |
| Gametocyte | expressed | 0.77 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0919400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.976 | MFS: -2.743 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0820300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0820300)
Modification: Tagged | RMgm-4711
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2V9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.3.4.1 | protein disulfide-isomerase | NEM16F16LOC14AsO43-CCF642MA3 018MA3 019acroleinneomycinpeptidesgenisteinkanamycin... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 23 | 21 | 38 |
| disruptive | 8 | 36 | 12 | 79 |
| missense | 8 | 36 | 12 | 76 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 53 | 27 | 38 |
| disruptive | 15 | 76 | 49 | 129 |
| missense | 14 | 73 | 47 | 99 |
PlasmoDB Total SNPs: 161
Non-coding: 143 | Synonymous: 13 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 515 | Molecular Weight (kDa): 61.001
UniProt ID(s): Q8I2V9
PDB ID(s): None
Isoelectric Point: 5.92
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013766 | Thioredoxin domain |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| PFam | PF00085 | Thioredoxin domain |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 31797966 | Male-Specific Protein Disulphide Isomerase Function is Essential for PlasmodiumTransmission and a Vulnerable Target for Intervention. | Angrisano F, Sala KA, ..., Blagborough AM | 10.1038/s41598-019-54613-0 |