PF3D7_0919500 (MFS3)

Genome location: Pf3D7_09_v3:798,498..799,928(+)

Genome classification: Core

Function and Localization

Product Description: major facilitator superfamily domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface
Component	GO:0005739	mitochondrion
Component	GO:0005886	plasma membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	nd	1.00
Trophozoite	not expressed	0.00	1.00
Schizont	not expressed	0.01	1.00
Gametocyte	expressed	0.01	0.99

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0919500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 1.392 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0820400)

Relative Growth Rate: 0.98 ± 0.10
Confidence: 5.95

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0823700)

Modification: Disrupted | RMgm-4769

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2V8

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533727

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	37	9	40
disruptive	19	18	16	44
missense	18	17	16	40

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	56	25	28
disruptive	22	45	33	92
missense	20	39	27	63

PlasmoDB Total SNPs: 34

Non-coding: 0 | Synonymous: 14 | Nonsynonymous: 19 | Stop Codon: 1

Protein Information

Protein Length: 476 | Molecular Weight (kDa): 56.072

UniProt ID(s): Q8I2V8

PDB ID(s): None

Isoelectric Point: 8.48

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005828	Major facilitator, sugar transporter-like
InterPro	IPR036259	MFS transporter superfamily
PFam	PF00083	Major facilitator, sugar transporter-like
Superfamily	SSF103473	MFS transporter superfamily

Associated Publications

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
23325771	Total and putative surface proteomics of malaria parasite salivary glandsporozoites.	Lindner SE, Swearingen KE, ..., Kappe SH	10.1074/mcp.M112.024505
27128092	Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics.	Swearingen KE, Lindner SE, ..., Sinnis P	10.1371/journal.ppat.1005606
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21