Product Description: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 2.4.1.119 (Transferred entry: 2.4.99.18);2.4.99.18 (Dolichyl-diphosphooligosaccharide--protein glycotransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0008250 | oligosaccharyltransferase complex |
| Function | GO:0004579 | dolichyl-diphosphooligosaccharide-protein glycotransferase activity |
| Process | GO:0018279 | protein N-linked glycosylation via asparagine |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.56 | 0.68 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.73 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0919600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.137 | MFS: -3.079 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2V7
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.1.119 | dolichyl-diphosphooligosaccharideprotein glycotransferase | No BRENDA inhibitors |
| 2.4.99.18 | dolichyl-diphosphooligosaccharide-protein glycotransferase | EDTARNAiDigitoninoctylglucosideN-benzoyl-Asn-Leu-ThrN-octanoyl-Asn-Leu-ThrN-t-butoxycarbonyl-Asn-Leu-ThrArg-Asn-Gly-(Gly-allyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-vinyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-allylepoxide)-Ala-Val-methyl esterN-dinitrobenzoyl-Arg-Asn-Ala-epoxyethylglycine-Ala-ValArg-Asn-Gly-(Gly-epoxyethylglycine)-Ala-Val-methylester... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P39656 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit | Homo sapiens | OMA | CID44328462CID44328460CID44328456CHEMBL2370267... |
Ortholog Group (OrthoMCL): OG6_103031
Most Similar Human Ortholog: A0A0C4DGS1
TM-align score: 0.77 | RMSD: 3.51
Seq Identity: 0.21 | Length: 390 / 477
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000244038 | dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit |
Protein Length: 477 | Molecular Weight (kDa): 57.008
UniProt ID(s): Q8I2V7
PDB ID(s): None
Isoelectric Point: 10.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005013 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48kDa subunit |
| PFam | PF03345 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48kDa subunit |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 26 | 19 | 37 |
| disruptive | 1 | 48 | 34 | 66 |
| missense | 1 | 47 | 32 | 63 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 62 | 23 | 31 |
| disruptive | 9 | 107 | 55 | 104 |
| missense | 6 | 90 | 48 | 78 |
PlasmoDB Total SNPs: 74
Non-coding: 58 | Synonymous: 10 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |