PF3D7_0919700

Genome location: Pf3D7_09_v3:802,655..804,294(+)

Genome classification: Core

Function and Localization

Product Description: pyridoxal phosphate homeostasis protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.1.1.1 (Alanine racemase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0030170	pyridoxal phosphate binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.06	0.97
Trophozoite	expressed	0.17	0.90
Schizont	not expressed	0.01	0.99
Gametocyte	not expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0919700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.901 | MFS: -1.732 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2V6

"Best" AlphaFill ligand hit: PLP (pyridoxal-5'-phosphate, Local RMSD=0.17) with 7UB4 (Global RMSD=5.08)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.1.1.1	alanine racemase	FADNEMDTTPCMBNaBH4acetateglycinealaninepatulinpyruvateButyratesuccinate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101083

Most Similar Human Ortholog: E5RFX7

TM-align score: 0.91 | RMSD: 1.28

Seq Identity: 0.37 | Length: 132 / 242

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000147471	pyridoxal phosphate binding protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	14	8	20
disruptive	6	20	13	24
missense	6	20	13	22

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	26	12	18
disruptive	10	46	26	61
missense	10	41	21	44

PlasmoDB Total SNPs: 51

Non-coding: 25 | Synonymous: 18 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 242 | Molecular Weight (kDa): 28.395

UniProt ID(s): Q8I2V6

PDB ID(s): None

Isoelectric Point: 8.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011078	Pyridoxal phosphate homeostasis protein
InterPro	IPR029066	PLP-binding barrel
PFam	N/A	N/A
Superfamily	SSF51419	PLP-binding barrel