PF3D7_0920000 (ELO3)

Genome location: Pf3D7_09_v3:822,634..825,411(+)

Genome classification: Core

Function and Localization

Product Description: elongation of fatty acids protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: 2.3.1.199 (Very-long-chain 3-oxoacyl-CoA synthase);6.2.1.3 (Long-chain-fatty-acid--CoA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Component	GO:0030176	integral component of endoplasmic reticulum membrane
Function	GO:0009922	fatty acid elongase activity
Process	GO:0034625	fatty acid elongation, monounsaturated fatty acid
Process	GO:0034626	fatty acid elongation, polyunsaturated fatty acid
Process	GO:0019367	fatty acid elongation, saturated fatty acid
Process	GO:0030148	sphingolipid biosynthetic process
Process	GO:0042761	very long-chain fatty acid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	expressed	0.13	0.92
Schizont	expressed	0.06	0.96
Gametocyte	expressed	0.69	0.63

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0920000

Resistome Missense Mutations: K447N

Resistome Compounds with Missense Mutations: GNF707

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -1.138 | #Insertions: 2

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1446500)

Relative Growth Rate: 0.05 ± 0.05
Confidence: 7.48

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0820900)

Modification: Disrupted | RMgm-4605

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H545

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.199	very-long-chain 3-oxoacyl-CoA synthase	mefluidideperfluidonemetazachlor
6.2.1.3	long-chain-fatty-acid-CoA ligase	ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_110457

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	31	29	45
disruptive	17	65	29	96
missense	15	60	25	81

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	73	44	54
disruptive	30	145	61	167
missense	28	118	51	116

PlasmoDB Total SNPs: 107

Non-coding: 22 | Synonymous: 62 | Nonsynonymous: 23 | Stop Codon: 0

Protein Information

Protein Length: 642 | Molecular Weight (kDa): 75.491

UniProt ID(s): C0H545

PDB ID(s): None

Isoelectric Point: 9.13

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002076	ELO family
PFam	PF01151	ELO family
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
22179608	Apicoplast and endoplasmic reticulum cooperate in fatty acid biosynthesis inapicomplexan parasite Toxoplasma gondii.	Ramakrishnan S, Docampo MD, ..., Striepen B	10.1074/jbc.M111.310144
37061002	Fatty acid elongases 1-3 have distinct roles in mitochondrial function, growth,and lipid homeostasis in Trypanosoma cruzi	Pagura L, Dumoulin PC, ..., Burleigh BA	10.1016/j.jbc.2023.104715
28745545	A link between very long chain fatty acid elongation and mating-specific yeastcell cycle arrest	Villasmil ML, Gallo-Ebert C, ..., Nickels JT Jr	10.1080/15384101.2017.1329065
29458843	A critical role for very long-chain fatty acid elongases in oleic acid-mediatedSaccharomyces cerevisiae cytotoxicity	Wang Q, Du X, ..., Huang Z	10.1016/j.micres.2017.11.001