PF3D7_0920700

Genome location: Pf3D7_09_v3:848,626..851,420(+)

Genome classification: Core

Function and Localization

Product Description: CRAL/TRIO domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0045177	apical part of cell
Function	GO:0008526	phosphatidylinositol transfer activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.12	0.94
Trophozoite	not expressed	0.23	0.86
Schizont	expressed	1.10	0.49
Gametocyte	possibly expressed	0.39	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0920700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -2.969 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0821600)

Relative Growth Rate: 0.81 ± 0.13
Confidence: 5.43

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0821600)

Modification: Disrupted | RMgm-2393

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2U6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533343

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	28	19	32
disruptive	37	61	31	93
missense	24	48	28	77

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	52	29	40
disruptive	58	154	74	156
missense	37	114	59	115

PlasmoDB Total SNPs: 93

Non-coding: 28 | Synonymous: 40 | Nonsynonymous: 25 | Stop Codon: 0

Protein Information

Protein Length: 593 | Molecular Weight (kDa): 70.603

UniProt ID(s): Q8I2U6

PDB ID(s): None

Isoelectric Point: 6.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001251	CRAL-TRIO lipid binding domain
InterPro	IPR036273	CRAL/TRIO, N-terminal domain superfamily
InterPro	IPR036865	CRAL-TRIO lipid binding domain superfamily
PFam	PF00650	CRAL-TRIO lipid binding domain
Superfamily	SSF46938	CRAL/TRIO, N-terminal domain superfamily
Superfamily	SSF52087	CRAL-TRIO lipid binding domain superfamily

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
20037583	Transcriptional profiling of growth perturbations of the human malaria parasitePlasmodium falciparum.	Hu G, Cabrera A, ..., Bozdech Z	10.1038/nbt.1597