Product Description: U4/U6 snRNA-associated-splicing factor, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1990904 | ribonucleoprotein complex |
| Function | GO:0003723 | RNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.53 | 0.75 |
| Trophozoite | expressed | 1.16 | 0.43 |
| Schizont | possibly expressed | 0.30 | 0.81 |
| Gametocyte | possibly expressed | 0.85 | 0.55 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0920900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -2.956 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0821800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2U4
"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.11) with 1H2U (Global RMSD=4.36)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 36 | 12 | 34 |
| disruptive | 12 | 45 | 34 | 108 |
| missense | 12 | 44 | 34 | 100 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 57 | 23 | 29 |
| disruptive | 24 | 131 | 75 | 149 |
| missense | 18 | 113 | 66 | 111 |
PlasmoDB Total SNPs: 166
Non-coding: 126 | Synonymous: 25 | Nonsynonymous: 15 | Stop Codon: 0
Protein Length: 480 | Molecular Weight (kDa): 56.65
UniProt ID(s): Q8I2U4
PDB ID(s): None
Isoelectric Point: 9.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000504 | RNA recognition motif domain |
| InterPro | IPR035979 | RNA-binding domain superfamily |
| PFam | PF00076 | RNA recognition motif domain |
| Superfamily | SSF54928 | RNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 29615482 | Defining essential elements and genetic interactions of the yeast Lsm2-8 ring and demonstration that essentiality of Lsm2-8 is bypassed via overexpression of U6snRNA or the U6 snRNP subunit Prp24 | Roth AJ, Shuman S, Schwer B | 10.1261/rna.066175.118 |
| 19693704 | (1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA | Martin-Tumasz S, Butcher SE | 10.1007/s12104-009-9181-5 |
| 24837192 | Core structure of the U6 small nuclear ribonucleoprotein at 1.7-A resolution | Montemayor EJ, Curran EC, ..., Brow DA | 10.1038/nsmb.2832 |
| 20181740 | Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24 | Martin-Tumasz S, Reiter NJ, Brow DA, Butcher SE | 10.1261/rna.1913310 |
| 29717126 | Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6snRNA | Montemayor EJ, Didychuk AL, ..., Butcher SE | 10.1038/s41467-018-04145-4 |
| 26673715 | Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly | Didychuk AL, Montemayor EJ, Brow DA, Butcher SE | 10.1093/nar/gkv1374 |
| 25762536 | Spliceosome assembly in the absence of stable U4/U6 RNA pairing | Burke JE, Butcher SE, Brow DA | 10.1261/rna.048421.114 |
| 18971323 | Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy | Karaduman R, Dube P, ..., Luhrmann R | 10.1261/rna.1369808 |
| 15811912 | The N- and C-terminal RNA recognition motifs of splicing factor Prp24 have distinct functions in U6 RNA binding | Kwan SS, Brow DA | 10.1261/rna.2010905 |
| 16410014 | RNA structure and RNA-protein interactions in purified yeast U6 snRNPs | Karaduman R, Fabrizio P, ..., Luhrmann R | 10.1016/j.jmb.2005.12.013 |