PF3D7_0921000

Genome location: Pf3D7_09_v3:857,262..859,606(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-conjugating enzyme E2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.2.2 (Gamma-glutamyltransferase);2.3.2.23 (E2 ubiquitin-conjugating enzyme)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0061631	ubiquitin conjugating enzyme activity
Process	GO:0000209	protein polyubiquitination
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	nd	nd
Trophozoite	expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0921000.2

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.583 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZY34

"Best" AlphaFill ligand hit: U94 (, Local RMSD=1.52) with 6NYO (Global RMSD=5.11)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.2.2	gamma-glutamyltransferase	GSHDTTEDTANH4+EGTAPMSFGSSGL-SerL-MetGGsTopglycineMaleate...
2.3.2.23	E2 ubiquitin-conjugating enzyme	CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione...
2.3.2.2	gamma-glutamyltransferase	GSHDTTEDTANH4+EGTAPMSFGSSGL-SerL-MetGGsTopglycineMaleate...
2.3.2.23	E2 ubiquitin-conjugating enzyme	CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101758

Most Similar Human Ortholog: P62253

TM-align score: 0.95 | RMSD: 1.29

Seq Identity: 0.51 | Length: 162 / 163

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000132388	ubiquitin conjugating enzyme E2 G1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	7	5	9
disruptive	1	1	0	7
missense	1	1	0	5

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	24	8	9
disruptive	2	10	18	34
missense	1	5	12	22

PlasmoDB Total SNPs: 142

Non-coding: 136 | Synonymous: 6 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 163 | Molecular Weight (kDa): 18.688

UniProt ID(s): Q8I2U3

PDB ID(s): None

Isoelectric Point: 5.25

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000608	Ubiquitin-conjugating enzyme E2
InterPro	IPR016135	Ubiquitin-conjugating enzyme/RWD-like
InterPro	IPR023313	Ubiquitin-conjugating enzyme, active site
PFam	PF00179	Ubiquitin-conjugating enzyme E2
Superfamily	SSF54495	Ubiquitin-conjugating enzyme/RWD-like