Product Description: NifU-like scaffold protein
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Component | GO:0005739 | mitochondrion |
Function | GO:0051539 | 4 iron, 4 sulfur cluster binding |
Function | GO:0005506 | iron ion binding |
Process | GO:0016226 | iron-sulfur cluster assembly |
Process | GO:0097428 | protein maturation by iron-sulfur cluster transfer |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | not expressed | 0.01 | 1.00 |
Trophozoite | possibly expressed | 0.05 | 0.96 |
Schizont | possibly expressed | 0.00 | 1.00 |
Gametocyte | not expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0921400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.217 | MFS: -3.183 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0822300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0822300)
Modification: Disrupted | RMgm-2397
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2T9
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 3 | 15 | 7 | 23 |
disruptive | 4 | 21 | 18 | 40 |
missense | 4 | 21 | 18 | 39 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 5 | 30 | 12 | 22 |
disruptive | 9 | 58 | 40 | 66 |
missense | 7 | 55 | 30 | 44 |
PlasmoDB Total SNPs: 26
Non-coding: 17 | Synonymous: 2 | Nonsynonymous: 7 | Stop Codon: 0
Protein Length: 247 | Molecular Weight (kDa): 29.104
UniProt ID(s): Q8I2T9
PDB ID(s): None
Isoelectric Point: 7.06
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR001075 | NIF system FeS cluster assembly, NifU, C-terminal |
InterPro | IPR034904 | Fe-S cluster assembly domain superfamily |
PFam | PF01106 | NIF system FeS cluster assembly, NifU, C-terminal |
Superfamily | SSF117916 | Fe-S cluster assembly domain superfamily |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
23805304 | Experimental Genetics of Plasmodium berghei NFU in the Apicoplast Iron-SulfurCluster Biogenesis Pathway. | Haussig JM, Matuschewski K, Kooij TW | 10.1371/journal.pone.0067269 |
30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
36937264 | Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by usingpolyprotein strategy | Wang M, Shang Y, Liu X, Chen S | 10.3389/fmicb.2023.1137355 |