About Gene List

PF3D7_0921400 (NifU)

Genome location: Pf3D7_09_v3:873,971..875,133(-)

Genome classification: Core

Function and Localization

Product Description: NifU-like scaffold protein

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Component GO:0005739 mitochondrion
Function GO:0051539 4 iron, 4 sulfur cluster binding
Function GO:0005506 iron ion binding
Process GO:0016226 iron-sulfur cluster assembly
Process GO:0097428 protein maturation by iron-sulfur cluster transfer

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.01 1.00
Trophozoite possibly expressed 0.05 0.96
Schizont possibly expressed 0.00 1.00
Gametocyte not expressed 0.04 0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0921400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.217 | MFS: -3.183 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0822300)

  • Relative Growth Rate: 0.92 ± 0.09
  • Confidence: 6.27

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0822300)

Modification: Disrupted | RMgm-2397

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2T9

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_121363

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 15 7 23
disruptive 4 21 18 40
missense 4 21 18 39

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 5 30 12 22
disruptive 9 58 40 66
missense 7 55 30 44

PlasmoDB Total SNPs: 26

Non-coding: 17 | Synonymous: 2 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 247 | Molecular Weight (kDa): 29.104

UniProt ID(s): Q8I2T9

PDB ID(s): None

Isoelectric Point: 7.06

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001075 NIF system FeS cluster assembly, NifU, C-terminal
InterPro IPR034904 Fe-S cluster assembly domain superfamily
PFam PF01106 NIF system FeS cluster assembly, NifU, C-terminal
Superfamily SSF117916 Fe-S cluster assembly domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
23805304 Experimental Genetics of Plasmodium berghei NFU in the Apicoplast Iron-SulfurCluster Biogenesis Pathway. Haussig JM, Matuschewski K, Kooij TW 10.1371/journal.pone.0067269
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895
36937264 Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by usingpolyprotein strategy Wang M, Shang Y, Liu X, Chen S 10.3389/fmicb.2023.1137355