Product Description: S-adenosylmethionine synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.5.1.6 (Methionine adenosyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0009986 | cell surface |
| Component | GO:0005829 | cytosol |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0004478 | methionine adenosyltransferase activity |
| Function | GO:0005515 | protein binding |
| Function | GO:0042803 | protein homodimerization activity |
| Process | GO:0006556 | S-adenosylmethionine biosynthetic process |
| Process | GO:0045454 | cell redox homeostasis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.54 | 0.74 |
| Trophozoite | expressed | 1.26 | 0.41 |
| Schizont | expressed | 0.84 | 0.56 |
| Gametocyte | expressed | 0.34 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0922200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.207 | MFS: -2.73 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0823100)
Modification: Mutated | RMgm-5315
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7K6A4
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.10) with 5T8S (Global RMSD=1.33)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.6 | methionine adenosyltransferase | NOMnATPADPAMPGTPGDPCTPUTPGSHTTPEDTA... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P31153 | S-adenosylmethionine synthase isoform type-2 | OMA, OrthoDB | US11084798, Cpd No 2US11084798, Cpd No 36US11084798, Cpd No 70US11084798, Cpd No 3... | |
| B1LDF1 | S-adenosylmethionine synthase | Escherichia coli | OMA | FIDAS-3FIDAS-5 |
| P18298 | S-adenosylmethionine synthase isoform type-2 | Rattus norvegicus | OrthoDB | Epoxy analogue, I(a)AMP.P[NH]P |
| P13444 | S-adenosylmethionine synthase isoform type-1 | Rattus norvegicus | OrthoDB | CHEMBL611854CHEMBL3706402CHEMBL611854CHEMBL3706402... |
Ortholog Group (OrthoMCL): OG6_100388
Most Similar Human Ortholog: P31153
TM-align score: 0.97 | RMSD: 0.83
Seq Identity: 0.58 | Length: 388 / 402
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151224 | methionine adenosyltransferase 1A |
| ENSG00000168906 | methionine adenosyltransferase 2A |
Protein Length: 402 | Molecular Weight (kDa): 44.844
PDB ID(s): None
Isoelectric Point: 6.71
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002133 | S-adenosylmethionine synthetase |
| InterPro | IPR022628 | S-adenosylmethionine synthetase, N-terminal |
| InterPro | IPR022629 | S-adenosylmethionine synthetase, central domain |
| InterPro | IPR022630 | S-adenosylmethionine synthetase, C-terminal |
| InterPro | IPR022631 | S-adenosylmethionine synthetase, conserved site |
| InterPro | IPR022636 | S-adenosylmethionine synthetase superfamily |
| PFam | PF00438 | S-adenosylmethionine synthetase, N-terminal |
| PFam | PF02772 | S-adenosylmethionine synthetase, central domain |
| PFam | PF02773 | S-adenosylmethionine synthetase, C-terminal |
| Superfamily | SSF55973 | S-adenosylmethionine synthetase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 1 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 1 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 351
Non-coding: 332 | Synonymous: 19 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10567242 | Molecular characterization of Plasmodium falciparum S-adenosylmethioninesynthetase. | Chiang PK, Chamberlin ME, ..., Chou JY | https://pubmed.ncbi.nlm.nih.gov/10567242/ |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 27466371 | Characterization and redox regulation of Plasmodium falciparum methionineadenosyltransferase. | Pretzel J, Gehr M, ..., Jortzik E | 10.1093/jb/mvw045 |
| 27714937 | PlasmoSEP: Predicting surface-exposed proteins on the malaria parasite usingsemisupervised self-training and expert-annotated data. | El-Manzalawy Y, Munoz EE, Lindner SE, Honavar V | 10.1002/pmic.201600249 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 32310999 | Insights into the intracellular localization, protein associations andartemisinin resistance properties of Plasmodium falciparum K13. | Gnadig NF, Stokes BH, ..., Fidock DA | 10.1371/journal.ppat.1008482 |
| 38417836 | A long noncoding RNA functions in pumpkin fruit development through S-adenosyl-L-methionine synthetase | Tian J, Zhang F, ..., Li H | 10.1093/plphys/kiae099 |
| 38361361 | Mitochondrial S-adenosylmethionine deficiency induces mitochondrial unfolded protein response and extends lifespan in Caenorhabditis elegans | Chen TY, Wang FY, ..., Ching TT | 10.1111/acel.14103 |