PF3D7_0922400 (pBAS)

Genome location: Pf3D7_09_v3:908,583..912,061(+)

Genome classification: Core

Function and Localization

Product Description: para-aminobenzoic acid synthetase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.6.1.85 (Aminodeoxychorismate synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0046820	4-amino-4-deoxychorismate synthase activity
Process	GO:0008153	para-aminobenzoic acid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	expressed	0.24	0.86
Schizont	possibly expressed	0.07	0.96
Gametocyte	expressed	0.29	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0922400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.623 | #Insertions: 8

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0823300)

Relative Growth Rate: 0.47 ± 0.45
Confidence: 2.98

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0823300)

Modification: Tagged | RMgm-4596

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I2T0

"Best" AlphaFill ligand hit: BEZ (benzoic acid, Local RMSD=0.43) with 7BVD (Global RMSD=2.12)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.6.1.85	aminodeoxychorismate synthase	NH3phosphatepyocyanin2-fluorochorismate1-hydroxyphenazineatrop-abyssomicin C(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106370

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	43	25	55
disruptive	9	56	34	109
missense	9	55	34	100

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	84	35	62
disruptive	20	136	79	205
missense	18	125	73	146

PlasmoDB Total SNPs: 146

Non-coding: 81 | Synonymous: 47 | Nonsynonymous: 18 | Stop Codon: 0

Protein Information

Protein Length: 979 | Molecular Weight (kDa): 116.536

UniProt ID(s): Q8I2T0, Q9Y1K0

PDB ID(s): None

Isoelectric Point: 7.42

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005801	ADC synthase
InterPro	IPR015890	Chorismate-utilising enzyme, C-terminal
InterPro	IPR017926	Glutamine amidotransferase
InterPro	IPR029062	Class I glutamine amidotransferase-like
PFam	PF00117	Glutamine amidotransferase
PFam	PF00425	Chorismate-utilising enzyme, C-terminal
Superfamily	SSF52317	Class I glutamine amidotransferase-like
Superfamily	SSF56322	ADC synthase