Product Description: phosphoglycerate kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.2.3 (Phosphoglycerate kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0009986 | cell surface |
| Component | GO:0005829 | cytosol |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0043531 | ADP binding |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004618 | phosphoglycerate kinase activity |
| Process | GO:0006094 | gluconeogenesis |
| Process | GO:0006096 | glycolytic process |
| Process | GO:1903862 | positive regulation of oxidative phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.11 | 0.56 |
| Trophozoite | expressed | 2.25 | 0.17 |
| Schizont | expressed | 0.33 | 0.81 |
| Gametocyte | expressed | 0.65 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0922500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.141 | MFS: -3.358 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0823400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: P27362
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.19) with 1LTK (Global RMSD=0.57)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.2.3 | phosphoglycerate kinase | ATPADPUDPAMPGTPCTPUTPSDSGMPITPIMPIb1... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P00558 | Phosphoglycerate kinase 1 | HOGENOM, OMA, OrthoDB | CHEMBL356655CHEMBL356436CHEMBL347337CHEMBL149129... |
Ortholog Group (OrthoMCL): OG6_100440
Most Similar Human Ortholog: P07205
TM-align score: 0.98 | RMSD: 1.01
Seq Identity: 0.59 | Length: 415 / 416
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102144 | phosphoglycerate kinase 1 |
| ENSG00000170950 | phosphoglycerate kinase 2 |
Protein Length: 416 | Molecular Weight (kDa): 45.427
UniProt ID(s): P27362
PDB ID(s): 1LGI, 1LTK, 3OZ7, 3OZA
Isoelectric Point: 7.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001576 | Phosphoglycerate kinase |
| InterPro | IPR015911 | Phosphoglycerate kinase, conserved site |
| InterPro | IPR036043 | Phosphoglycerate kinase superfamily |
| PFam | PF00162 | Phosphoglycerate kinase |
| Superfamily | SSF53748 | Phosphoglycerate kinase superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 15 | 12 | 24 |
| disruptive | 2 | 9 | 8 | 35 |
| missense | 2 | 9 | 8 | 28 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 48 | 16 | 17 |
| disruptive | 3 | 41 | 32 | 71 |
| missense | 2 | 32 | 27 | 47 |
PlasmoDB Total SNPs: 136
Non-coding: 126 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 21798238 | Crystal structure of Plasmodium falciparum phosphoglycerate kinase: evidence foranion binding in the basic patch. | Smith CD, Chattopadhyay D, Pal B | 10.1016/j.bbrc.2011.07.045 |
| 27714937 | PlasmoSEP: Predicting surface-exposed proteins on the malaria parasite usingsemisupervised self-training and expert-annotated data. | El-Manzalawy Y, Munoz EE, Lindner SE, Honavar V | 10.1002/pmic.201600249 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 38162673 | Immunoproteomics reveal increased serum IgG3/5 binding to Dermatophagoides andyeast protein antigens in severe equine asthma in a preliminary study | Schnabel CL, Jentsch MC, ..., Volke D | 10.3389/fimmu.2023.1293684 |