Product Description: glutamine synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.1.2 (Glutamine synthetase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Function | GO:0004356 | glutamate-ammonia ligase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.67 | 0.68 |
| Trophozoite | possibly expressed | 0.47 | 0.72 |
| Schizont | expressed | 0.15 | 0.90 |
| Gametocyte | possibly expressed | 0.62 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0922600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -2.576 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0823500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0823500)
Modification: Disrupted | RMgm-2402
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H551
"Best" AlphaFill ligand hit: PPQ (2-amino-4-(hydroxymethyl-phosphinyl)butanoic acid, Local RMSD=0.04) with 1FPY (Global RMSD=6.48)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.1.2 | glutamine synthetase | ATPADPAMPGDPCTPUTPUMPGlyITPAspAlaLeu... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P0A9C5 | Glutamine synthetase | Escherichia coli | OMA, OrthoMCL, OrthoMCL BLAST | CHEMBL536046CHEMBL536046CHEMBL535377CHEMBL535153... |
Ortholog Group (OrthoMCL): OG6_100656
Most Similar Human Ortholog: Q5TDP6
TM-align score: 0.74 | RMSD: 3.46
Seq Identity: 0.20 | Length: 423 / 543
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000146166 | lengsin, lens protein with glutamine synthetase domain |
Protein Length: 543 | Molecular Weight (kDa): 63.231
UniProt ID(s): C0H551
PDB ID(s): None
Isoelectric Point: 6.54
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008146 | Glutamine synthetase, catalytic domain |
| InterPro | IPR008147 | Glutamine synthetase, beta-Grasp domain |
| InterPro | IPR014746 | Glutamine synthetase/guanido kinase, catalytic domain |
| InterPro | IPR027303 | Glutamine synthetase, glycine-rich site |
| InterPro | IPR036651 | Glutamine synthetase, N-terminal domain superfamily |
| PFam | PF00120 | Glutamine synthetase, catalytic domain |
| PFam | PF03951 | Glutamine synthetase, beta-Grasp domain |
| Superfamily | SSF54368 | Glutamine synthetase, N-terminal domain superfamily |
| Superfamily | SSF55931 | Glutamine synthetase/guanido kinase, catalytic domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 31 | 11 | 24 |
| disruptive | 2 | 18 | 11 | 38 |
| missense | 2 | 18 | 10 | 35 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 61 | 17 | 24 |
| disruptive | 6 | 60 | 40 | 109 |
| missense | 5 | 46 | 30 | 58 |
PlasmoDB Total SNPs: 115
Non-coding: 81 | Synonymous: 29 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |