Product Description: pre-mRNA-splicing factor 18, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071021 | U2-type post-spliceosomal complex |
| Component | GO:0046540 | U4/U6 x U5 tri-snRNP complex |
| Component | GO:0005682 | U5 snRNP |
| Component | GO:0005634 | nucleus |
| Process | GO:0000350 | generation of catalytic spliceosome for second transesterification step |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
| Process | GO:0071048 | nuclear retention of unspliced pre-mRNA at the site of transcription |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.22 | 0.89 |
| Trophozoite | expressed | 0.40 | 0.76 |
| Schizont | expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.44 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0922700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.129 | MFS: -3.198 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0823600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I2S9
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102077
Most Similar Human Ortholog: Q99633
TM-align score: 0.45 | RMSD: 3.72
Seq Identity: 0.32 | Length: 181 / 343
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000165630 | pre-mRNA processing factor 18 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 16 | 9 | 29 |
| disruptive | 1 | 17 | 16 | 36 |
| missense | 1 | 17 | 16 | 33 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 33 | 17 | 25 |
| disruptive | 6 | 59 | 26 | 77 |
| missense | 3 | 45 | 21 | 48 |
PlasmoDB Total SNPs: 180
Non-coding: 152 | Synonymous: 25 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 343 | Molecular Weight (kDa): 40.558
UniProt ID(s): Q8I2S9
PDB ID(s): None
Isoelectric Point: 9.38
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004098 | Prp18 |
| InterPro | IPR014906 | Pre-mRNA processing factor 4 (PRP4)-like |
| InterPro | IPR036285 | PRP4-like superfamily |
| InterPro | IPR039979 | Pre-mRNA-splicing factor 18 |
| PFam | PF02840 | Prp18 |
| PFam | PF08799 | Pre-mRNA processing factor 4 (PRP4)-like |
| Superfamily | SSF158230 | PRP4-like superfamily |
| Superfamily | SSF47938 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 27875300 | The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specificSplicing Functions with Links to G1-S Cell Cycle Progression | Vijaykrishna N, Melangath G, ..., Vijayraghavan U | 10.1074/jbc.M116.751289 |
| 28919079 | Structure of an Intron Lariat Spliceosome from Saccharomyces cerevisiae | Wan R, Yan C, ..., Shi Y | 10.1016/j.cell.2017.08.029 |
| 27980089 | Structure of a yeast step II catalytically activated spliceosome | Yan C, Wan R, ..., Shi Y | 10.1126/science.aak9979 |
| 24722551 | Widespread use of non-productive alternative splice sites in Saccharomycescerevisiae | Kawashima T, Douglass S, ..., Chanfreau GF | 10.1371/journal.pgen.1004249 |
| 23685439 | Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigatedin a purified system | Ohrt T, Odenwalder P, ..., Luhrmann R | 10.1261/rna.039024.113 |